畜牧兽医学报2017，Vol.48Issue(5)：836-845,10.DOI:10.11843/j.issn.0366-6964.2017.05.007
水牛卵母细胞GV/MⅡ期对应卵丘细胞miRNA差异表达分析
Characterization of the Expression of miRNAs in Cumulus Cells during GV/M Ⅱ Stages of Buffalo Oocyte
摘要
Abstract
The aim of this study was to establish the miRNA database of cumulus cells in GV/M Ⅱ stages of buffalo oocytes during maturation in vitro,and then analyze the differential-expression miRNAs of cumulus cells between GV and M Ⅱ stages of oocytes to provide scientific basis for further understanding the important role of cumulus cells in buffalo oocytes maturation.Total RNAs of cumulus cells in GV/M Ⅱ stages were extracted,respectively,and subjected to small RNA sequencing by Solexa sequencing,the miRNA data were then analyzed through bioinformatics.The results indicated that the miRNA expression profiles in GV/M Ⅱ cumulus cells were successfully constructed.There were 24 up-regulated and 45 down-regulated miRNAs in cumulus cells in M Ⅱ stage compared with those in GV stage.The expression profiles of 8 randomly selected differential-expression miRNAs examined by qRT-PCR was agreement with those by RNA sequencing.Total of 276 enriched signaling pathways related to the miRNA of the cumulus cells were analyzed by miRNA target gene prediction and KEGG enriched pathways.The top 20 enriched signaling pathways were summarized and we found that the differential-expression miRNAs played roles in cumulus mainly through cell proliferation and apoptosis,material metabolism,cell junction as well as other unknown pathways.关键词
水牛/卵丘细胞/miRNAs/Solexa测序/生物信息学Key words
buffalo/cumulus cells/miRNAs/Solexa sequencing/bioinformatics分类
农业科技引用本文复制引用
沈朋雷,陆凤花,秦希玲,尹倩,郭振伟,邓凯,杜姗姗,尹娜,阮子芸,石德顺..水牛卵母细胞GV/MⅡ期对应卵丘细胞miRNA差异表达分析[J].畜牧兽医学报,2017,48(5):836-845,10.基金项目
国家高技术研究发展计划“863”项目(2011AA100607) （2011AA100607）
国家自然科学基金项目(31560633 （）
31460282) （）
广西科学基金杰青项目(2012GXNSFFA060004) （2012GXNSFFA060004）
广西研究生教育创新计划项目(YCBZ2015004) （YCBZ2015004）
