畜牧兽医学报2017，Vol.48Issue(5)：846-853,8.DOI:10.11843/j.issn.0366-6964.2017.05.008
与脐带间充质干细胞共培养对乳腺上皮细胞乳脂合成及关键基因表达的影响
Effect of Co-culturing with Umbilical Cord Mesenchymal Stem Cells on Milk Fat Synthesis and Expression of Key Genes in Bovine Mammary Gland Epithelial Cells
摘要
Abstract
In order to explore the effect on milk fat synthesis and the expression of key genes of milk fat synthesis in bovine mammary gland epithelial cells co-cultured with umbilical cord mesenchymal stem cells,8 groups were divided in the study:UC-MSCs and BMECs co-cultured under the condition of no treatment group,IGF-1 R inhibitor AG1024 treatment group,Janus kinase activators of transcription (JAK/STAT) pathway inhibitor AG490 treatment group and AG1024-+-AG490 treatment group,control group:BMECs alone cultured under the condition of no treatment group,IGF-1 R inhibitor AG1024 group,Janus kinase and transcription activating factor (JAK/STAT) signal pathway AG490 group and AG1024+AG490 treatment group.The IGF-1,Triglyceride (TG) content of each group supernatant were determined,the relative expression abundance of Acetyl-CoA carboxylase A (ACACA),fatty acid synthetase (FASN) and sterol regulatory element binding proteins (SREBP 1) mRNA were detected by RT-PCR.Results showed that the IGF-1 and TG content of co-culture group were significantly higher than control group (P＜0.05);AG1024 treatment had a extremely significant effect on inhibiting IGF-1,and the TG content and the relative expression abundance of ACACA,FASN,SREBP 1 mRNA were significantly decreased(P＜0.05);AG490 had no significant effect on the relative expression abundance ofACACA,FASN,SREBP 1 mRNA (P＞0.05);AG1024 and AG490 co-treatment were not significant difference for each index than AG1024 treatment alone.(P＞0.05).In conclusion,umbilical cord mesenchymal stem cells can promote milk fat synthesis and the expression of key gene in bovine mammary gland epithelial cells via IGF-1,but JAK2/STAT5 signaling pathway is not involving in the regulation of umbilical cord mesenchymal stem cells on milk fat of bovine mammary gland epithelial cells.关键词
脐带间充质干细胞/乳腺上皮细胞/共培养/乳脂/基因表达Key words
umbilical cord mesenchymal stem cells/mammary epithelial cells/co-culture/milk fat/gene expression分类
农业科技引用本文复制引用
赵艳坤,邵伟,王立文,余雄..与脐带间充质干细胞共培养对乳腺上皮细胞乳脂合成及关键基因表达的影响[J].畜牧兽医学报,2017,48(5):846-853,8.基金项目
奶产业体系资助(CARS-37) （CARS-37）
国家自然科学基金(31560645) （31560645）
中国博士后基金 （）
新疆维吾尔自治区高等学校科研计划项目(XJEDU2013S17) （XJEDU2013S17）
2013年度新疆研究生科研创新项目(xjau-2013-yjsky-XJGRI2013113) （xjau-2013-yjsky-XJGRI2013113）
新疆肉乳用草食动物营养实验室开放课题 （）
