畜牧兽医学报2017，Vol.48Issue(5)：863-870,8.DOI:10.11843/j.issn.0366-6964.2017.05.010
限饲对湖羊子宫RGMb基因及BMP系统成员表达的影响
Effect of Food Restriction on the Expression of RGMb and BMP System Members in the Uterus of Hu Ewes
摘要
Abstract
The study aimed to explore the effects of food restriction on the expression of RGMb and BMP system members in the uterus of Hu ewes.Firstly,qPCR were applied to investigate the RGMb expression profile in different tissues of 3-month-old Hu ewes,IHC were used to detect the expression of RGMb in uterus.Then Hu ewes on 35 days of pregnancy (d35) were divided into 2 groups:ewes in the control (C) group were fed with 100％ NRC and ewes in the restriction (R) group were fed with 50％ NRC.On 110 days of pregnancy,uterine tissues (n=8) were collected to evaluate the expression of BMP2,BMP4,BMPR1A,BMPR1B,BMPR2 and RGMb by qPCR and WB.qPCR results showed that RGMb mRNA were detected in 14 tissues of 3-month-old Hu ewes,and the relative expression was greater than 0.5 in uterus.IHC staining showed that RGMb protein were mainly expressed in the endometrial luminal and glandular epithelial cells of Hu ewes.The uterine expression of RGMb protein in the R group was significantly lower (P＜0.05) than that in the C group,while there was no significant differences in RGMb mRNA abundance(P＞0.05).The expression of BMP4 and BMP2 mRNAs in the R group were higher (P＜ 0.05) than those in the C group.However,the expression of BMP receptors(BMPR1A,BMPR1B and BMPR2) significantly decreased in the R group(P＜0.05).The results indicate that food restriction affect the expression of BMP system members,including the RGMb,suggesting RGMb accompanied with BMP system may be involved in the regulation of uterine hemeostasis,which will set up experimental evidences for the further study on the regulation mechanism of BMP system in the uterus.关键词
RGMb/BMP/限饲/湖羊/子宫Key words
RGMb/BMP/food restriction/Hu ewes/uterus分类
农业科技引用本文复制引用
邝美倩,金鹏锦,王若丞,孙玲伟,王锋,李鹏,成志军,茆达干..限饲对湖羊子宫RGMb基因及BMP系统成员表达的影响[J].畜牧兽医学报,2017,48(5):863-870,8.基金项目
江苏省农业科技支撑计划(BE2014330) （BE2014330）
