畜牧兽医学报2017，Vol.48Issue(5)：896-906,11.DOI:10.11843/j.issn.0366-6964.2017.05.014
山羊副流感病毒3型感染MDBK细胞的miRNA表达谱变化分析
Identification and Analysis of the miRNA Expression Profiles in MDBK Cells in Response to the Infection of Caprine Parainfluenza Virus Type 3 (CPIV3)
摘要
Abstract
To explore the function of the cellular miRNAs in MDBK cells infected with caprine parainfluenza virus type 3 (CPIV3),we compared the miRNA expression profiles in normal MDBK cells and MDBK cells infected with 10 MOI CPIV3 for 24 hours using high-throughput sequencing.GO analysis of the host target genes and KEGG enrichment were performed.The resuits indicated that total 37 miRNAs were obviously differently expressed in the infected MDBK cells including 18 up-regulated miRNAs and 19 down-regulated miRNAs.The five of these miRNAs were verified by RT-qPCR,the results were well matched with those of sequencing data.GO analysis of the host target genes showed that these responded miRNAs were involved in immunoregulation,biological regulation and metabolism.KEGG enrichment analysis suggested that these genes functioned in various cellular signaling pathways,including B cell receptor signaling pathway,MAPK signaling pathway,metabolic pathways,focal adhesion,cell adhesion molecules,calcium signaling pathway.Our experiment provided the data to further explore the molecular mechanism of differential expression of cellular miRNA involved in CPIV3 pathogenesis.关键词
山羊副流感病毒3型/miRNA/MDBK细胞/高通量测序Key words
caprine parainfluenza virus type 3/miRNA/MDBK cells/high-throughput sequencing分类
农业科技引用本文复制引用
李基棕,李文良,毛立,郝飞,杨蕾蕾,张纹纹,江杰元..山羊副流感病毒3型感染MDBK细胞的miRNA表达谱变化分析[J].畜牧兽医学报,2017,48(5):896-906,11.基金项目
江苏省农业科技自主创新资金项目[CX(15)1061] （15）
国家自然科学基金(31402180) （31402180）
十三五国家重点研发计划项目(2016YFD0500908) （2016YFD0500908）
