畜牧兽医学报2017，Vol.48Issue(5)：907-913,7.DOI:10.11843/j.issn.0366-6964.2017.05.015
不同致病性H9N2亚型禽流感病毒诱导鸡TLR-7和Mx基因mRNA转录水平的差异
Transcriptional Responses of TLR-7 and Mx mRNA in 3 Chicken Host Systems Infected with H9N2 Influenza Viruses with Different Pathogenicities
摘要
Abstract
For a better understanding of the mechanism underlying the expression of type Ⅰ IFN induced by H9N2 influenza viruses with different pathogenicity,mRNA of TLR-7 and Mx were dynamically studied in 3 host systems of chicken which infected with strains SD/818 and SD/196 in this study.SPF chickens,SPF chicken embryos and primary chicken embryo fibroblasts were randomly divided into three groups,control group,SD/196-infected group and SD/818-infected group.The lungs,kidneys,duodenum,bursa of Fabricius,embryonic bodies and primary chicken embryo fibroblasts were collected at different time points after infection,and mRNA of TLR7 and Mx were dynamically studied using real-time quantitative reverse transcription PCR.The results showed that transcriptional level of TLR-7 and Mx in chicken host systems induced by SD/818 were significantly higher than those induced by SD/196 except expression of TLR-7 at chicken embryos.The results suggested that expression of TLR-7 and Mx mRNA in chicken host systems were induced by H9N2 influenza virus with different pathogenicity,and transcriptional level of TLR-7 and Mxc induced by SD/818 were higher than those induced by SD/196.关键词
H9N2亚型禽流感病毒/致病性/SPF鸡/TLR-7/MxKey words
H9N2 influenza viruses/pathogenicity/SPF chicken/TLR-7/Mx/expression分类
农业科技引用本文复制引用
王建琳,曹志伟,王冬冬,尹燕博..不同致病性H9N2亚型禽流感病毒诱导鸡TLR-7和Mx基因mRNA转录水平的差异[J].畜牧兽医学报,2017,48(5):907-913,7.基金项目
国家自然科学基金项目(31272535) （31272535）
山东省现代农业产业技术体系项目(SDAIT-11-03) （SDAIT-11-03）
山东省自然科学基金(ZR2013CL022) （ZR2013CL022）
