畜牧兽医学报2017，Vol.48Issue(5)：914-921,8.DOI:10.11843/j.issn.0366-6964.2017.05.016
cGAS介导牛分枝杆菌诱导小鼠髓源树突状细胞成熟
Cyclic GMP-AMP Synthase-mediated Maturation of Murine Bone Marrow Derived Dendritic Cells after Infection with Mycobacterium bovis
摘要
Abstract
The study was performed to investigate the regulation of cyclic GMP-AMP synthase (cGAS) on Mycobacterium bovis infected murine bone marrow derived dendritic cell (BMDC).The murine bone marrow cells were cultured and induced into DCs in vitro.Then BMDCs were infected by Mycobacterium bovis to establish infection models.Three groups were designed for the in vitro study.They were control group (BMDC control),infection group (M.bovis infected BMDC) and silence group.In silence group,BMDC cGAS gene was knocked down via siRNA and then cells were infected similar to infection group.Flow cytometry was used to analyze the expression of surface markers.In cGAS pathway,STING,TBK1,p-TBK1 and IRF3 were determined by Western blot and Immunofluorescence,and the cytokine production in cell supernatant was assessed by ELISA.The data showed that M.bovis induced higher expression levels of the surface markers CD86,CD80,CD40,MHC-Ⅱ and eytokine production IFN-β,TNF-α,IL-12p70,IL-6 in BMDC.Meanwhile,the cGAS pathway was activated and induced higher expression of related protein,however corresponding data reduced significantly in silence group.Our research indicated that cGAS pathway can be activated by Mycobacterium bovis when BMDC was infected,and cGAS contribute to maturation and activation of BMDC.关键词
小鼠髓源树突状细胞/cGAS/牛分枝杆菌/信号通路/细胞因子Key words
BMDC/cGAS/Mycobacterium bovis/signal pathway/cytokines分类
农业科技引用本文复制引用
李强,刘春法,何小丽,李凡飞,魏凡华,赵德明,周向梅,许立华..cGAS介导牛分枝杆菌诱导小鼠髓源树突状细胞成熟[J].畜牧兽医学报,2017,48(5):914-921,8.基金项目
国家自然科学基金(31560687 （）
31572487) （）
宁夏自然科学基金(NZ12104 （）
NZ15031) （）
