畜牧兽医学报2017，Vol.48Issue(5)：938-944,7.DOI:10.11843/j.issn.0366-6964.2017.05.019
单色绿光对鸡胚褪黑激素合成的影响
The Effect of Monochromatic Green Light on Melatonin Synthesis in Chick Embryo
摘要
Abstract
To explore the effects of monochromatic green light on chick pineal body development and melatonin secretion,120 chick embryos at weight of 65±3 g were selected and divided them into two groups randomly with one group hatched in monochromatic green light and the other in darkness.Both research groups conducted four repeat,each including five chick embryos,and relative tissue extracted from embryos at the embryonic age (E) of 15th,18th and 21st day,for observation,to fulfill the objective,so as to explore the effects of monochromatic green light.The result exhibits:①Chick embryos with monochromatic green light during incubation period had an increase in birth weight of 11.19％-21.41％ (P＜0.05);(②)Monochromatic green light promoted the development of pineal parenchymal lobules;③Expression of AANAT mRNA,key of melatonin synthesis increased as the embryos developed.Compared with E15 group and E18group form the darkness group,melatonin secretion of E21 group increased obviously by 29.84％-47.06％ (P＜0.05),however,no significant difference between E15 and E18;In green light group,the AANAT mRNA expression of E18 group was significant higher by 17.76％ than that of E15 (P＜0.05),E21 was significant higher by 26.37％-48.81％ than E15 and E18 (P＜0.05);Significant differences were found between green light group and the darkness group,green light group higher by 12.85％-15.95％ than the darkness group (E18-E21,P＜0.05);④Plasma melatonin increased as the embryo developed,in the darkness group,E21 was 2.6 times of E15 and 1.9 times of E18;In green light group,E21 equaled to 2.8 times of E15 and 1.8 times of E18;and for E18 and E21,plasma melatonin of green light group was higher than that of darkness group by 13.13％ and 18.20％ (P＜0.05);Plasma melatonin was associated with plasma growth hormone notably (P＜0.001).Providing monochromatic green light during incubation attributed to chick embryo's growth by promoting the synthesis and secretion of melatonin,and then promoted growth hormone,finally promoted chick embryo's development.关键词
单色绿光/褪黑激素/AANAT/松果体/生长激素/鸡胚Key words
monochromatic green light/melatonin/AANAT/pineal body/growth hormone/chick embryo分类
农业科技引用本文复制引用
逄金吉,白欣洁,陈顺琪,蒋无砚,王子旭,曹静,董玉兰,陈耀星..单色绿光对鸡胚褪黑激素合成的影响[J].畜牧兽医学报,2017,48(5):938-944,7.基金项目
国家自然科学基金(31172277 （）
31272516 （）
31472157 （）
31672501) （）
高等学校博士科学点专项科研基金(20130008110031) （20130008110031）
国家级大学生创新计划(201510019070) （201510019070）
