畜牧兽医学报2017，Vol.48Issue(5)：945-953,9.DOI:10.11843/j.issn.0366-6964.2017.05.020
(—)-HCA对鸡胚原代肝细胞增殖和线粒体功能的影响
The Effect of (—)-Hydroxycitric Acid on Proliferation and Mitochondria Function in Cultured Primary Chicken Hepatocytes
摘要
Abstract
The present study aimed to investigate the effects of (—)-Hydroxycitric acid (HCA) on cells proliferation,mitochondrial ultrastructure and function in primary chicken hepatocytes.The cells were treated with 0,1,10 and 50μmol · L-1 (—)-HCA and then detected the factors related with cells proliferation and mitochondria function.The results showed that (—)-HCA treatment promoted the cell proliferation in primary chicken hepatocytes.Tenμmol · L-1 (—)-HCA treatment significantly increased the S phase and G2/M phase cell population (P＜0.01),meanwhile it increased the cyclin mRNA expression level when compared to control group.No significantly changes were observed on the mitochondrial morphology,quantity and membrane potential in primary chicken hepatocytes following 1-50 μtmol · L-1 (—)-HCA treatment (P ＞ 0.05),while (—)-HCA treatment significantly increased the activities of succinate dehydrogenase and citrate synthase in primary chicken hepatocytes (P＜0.05).These results indicated that (—)-HCA treatment promote the primary chicken hepatocytes proliferation by increasing cyclin mRNA expression,whereas it increase the energy metabolism by enhancing the activities of succinate dehydrogenase and citrate synthase in primary chicken hepatocytes.关键词
(—)-HCA/鸡胚原代肝细胞/细胞周期/线粒体功能Key words
(—)-HCA/primary chicken hepatocytes/cell proliferation/mitochondrial function分类
农业科技引用本文复制引用
李龙龙,彭梦玲,王典,俞磊,葛重阳,马海田..(—)-HCA对鸡胚原代肝细胞增殖和线粒体功能的影响[J].畜牧兽医学报,2017,48(5):945-953,9.基金项目
国家自然科学基金(31572483) （31572483）
江苏省自然科学基金(BK20151434) （BK20151434）
