畜牧兽医学报2017，Vol.48Issue(10)：1815-1824,10.DOI:10.11843/j.issn.0366-6964.2017.10.004
TNFRSF1A基因CpG岛甲基化与基因表达及T淋巴细胞亚群指标的相关性分析
Correlation Analyses of CpG Island Methylation of TNFRSF1A with Gene Expression and T Lymphocyte Subpopulation Traits
摘要
Abstract
The aim of this study was to explore CpG islands methylation status of TNFRSF1A gene,and clarify the relationship of methylation level with its expression and T lymphocyte subpopulation traits.In the present study,Dapulian piglets,one indigenous breed with higher disease resistance in North China,and Landrace piglets,one commercial breed with lower disease resistance,were used.Methylation level of CpG islands of TNFRSF1A gene upstream was determined by bisulfite sequencing PCR (BSP),and correlation analyses of methylation frequency of CpG sites with its expression and T lymphocyte subpopulation traits were carried out.The resuits indicated that:1) There were 2 CpG islands in the upstream of TNFRSF1A gene,which contained 25 CpG sites by BSP overlapping 2 islands.Nine CpG sites were in Island 1,nine CpG sites were in Island 2,and others located between 2 islands.Population methylation frequency between Dapulian and Landrace piglets had no significant difference in Island 1 at 0.05 level,whereas methylation frequency of Dapulian piglets was significantly higher than that of Landrace piglets in Island 2 at 0.05 level.2) The methylation frequencies in Island 2 were negatively correlated with the expression of TNFRSF1A,especially 5 CpG sites (+126,+128,+159,+162 and +164).3) We also found methylation frequencies of CpG sites +159 and +164 were positively correlated with CD4+CD8+CD3+ at 0.05 level in the 2 populations.The methylation frequencies of CpG sites +159 and +164 in Island 2 were negatively correlated with the expression of TNFRSF1A,and positively correlated with T lymphocyte subpopulation trait CD4 +CD8+ CD3+.Thus,we speculated that increased methylation frequencies of CpG sites +159 and +164 might lead to the decreased expression of TNFRSF1A,which may influence on T lymphocyte subpopulation traits and the immunity of Dapulian piglets.关键词
TNFRSF1A/甲基化/T淋巴细胞亚群/相关性分析/大蒲莲仔猪Key words
TNFRSF1A/methylation/T lymphocyte subpopulation/correlation analyses/Dapulian piglets分类
农业科技引用本文复制引用
赵雪艳,王彦平,王怀中,郭建凤,齐波,王继英..TNFRSF1A基因CpG岛甲基化与基因表达及T淋巴细胞亚群指标的相关性分析[J].畜牧兽医学报,2017,48(10):1815-1824,10.基金项目
转基因生物新品种培育重大科技专项(2014ZX08009-045B) （2014ZX08009-045B）
山东省现代农业产业技术体系生猪创新团队建设项目(SDAIT-08-03) （SDAIT-08-03）
山东省农业科学院农业科技创新工程“畜禽种质资源保护与共享利用”(CXGC2016A04) （CXGC2016A04）
山东省中青年科学家科研奖励基金(ZR2016CB15) （ZR2016CB15）
