畜牧兽医学报2017，Vol.48Issue(10)：1825-1832,8.DOI:10.11843/j.issn.0366-6964.2017.10.005
CaN/NFAT信号通路相关基因在鸡骨骼肌中的表达规律及关联分析
The Expression Profile of CaN/NFAT-Dependent Pathway Genes and Their Correlation Analysis in Chicken Skeletal Muscle
摘要
Abstract
The objective of the present study was to investigate the expression profile of CaN/NFAT-dependent pathway genes,and their correlation analysis in 2 phenotypically distinct skeletal muscles were studied in chicken during the early development,qRT-PCR was used to detect the mRNA expression of CnAα,CnB1,NFATc3,MEF2C and PPARGC1A genes in lateral gastrocnemius and extensor digitorum longus of Recessive White chicken at 0,1,3,5,7 and 9 week age,the correlation among expression of these genes was analyzed.The results showed that the expression trends of all the genes between 2 phenotypically distinct skeletal muscles were similar except CnB1,and the expression level of these genes in 2 phenotypically distinct skeletal muscles was all raised from 7 to 9 week age.Comparison among the expression of these genes in 2 phenotypically distinct skeletal muscle showed that the expression level of PPARGC1A in the lateral gastrocnemius was higher than that in the extensor digitorum longus at each detected age,and the expression level of other 4 genes in the lateral gastrocnemius was higher at 3 week age and lower at 7 and 9 week age than that in the extensor digitorum longus.Correlation analysis among the 5 genes expression levels in the 2 phenotypically distinct skeletal muscles showed that there was a negative correlation between the CnAα and 2 genes (NFATc3 and PPARGC1A),and positive correlations among the expression of other genes.Overall,the expression patterns of CnAα,CnB1,NFATc3,MEF2C and PPARGC1A genes in chicken skeletal muscle are age and phenotype specific,which may be related to the regulation of the myofiber growth and type transition.关键词
鸡/肌纤维/CaN/NFAT信号通路/基因表达/相关性Key words
chicken/muscle fiber/CaN/NFAT signaling pathway/gene expression/correlation分类
农业科技引用本文复制引用
盛中伟,单艳菊,束婧婷,章明,姬改革,屠云洁,范建华,邹剑敏..CaN/NFAT信号通路相关基因在鸡骨骼肌中的表达规律及关联分析[J].畜牧兽医学报,2017,48(10):1825-1832,8.基金项目
国家自然科学基金(31301967 （）
31572358) （）
江苏省自然科学基金(BK20161322 （）
BK20151317) （）
现代农业产业技术体系建设专项资金(CARS-42) （CARS-42）
江苏省农业科技自主创新资金(CX(15)1009) （CX(15）
江苏省重点研发计划(现代农业)(BE2015344) （现代农业）
扬州市科技计划项目(YZ2014144) （YZ2014144）
