畜牧兽医学报2017，Vol.48Issue(10)：1949-1957,9.DOI:10.11843/j.issn.0366-6964.2017.10.018
番鸭呼肠孤病毒人工感染雏番鸭肝的蛋白质组学分析
Proteomics Analysis in Livers of Muscovy Ducklings Infected by Muscovy Duck Reovirus
摘要
Abstract
The purpose of this paper was to study the pathogenesis of Muscovy duck reovirus (MDRV) infected muscovy ducking.Forty-four healthy muscovy ducklings were divided into treatment group challenged with MDRV and control one injected with Hank's.Livers were collected at the 5th day after challenge.Proteomics of the livers were determined by differential proteomic technology.Differential protein spots between both groups were performed and analyzed by two-dimensional gel electrophoresis and MALDI-TOF-TOF mass spectrometer,and then they were identified by protein search software,Mascot 2.3.02.Thirty-eight differential protein spots were separated and identified.Ten down-regulated protein spots and seventeen up-regulated protein spots were observed in the liver samples of the treatment after comparison between the two groups.Seven protein spots were appeared only in the livers of control and four protein spots were only emerged in the livers of treatment.The results indicate that differential proteomics in livers,which were impacted and damaged by MDRV may provide a new idea of pathogenesis of MDRV infected muscovy duckling.关键词
番鸭呼肠孤病毒/人工感染/番鸭/肝/蛋白质组学分析Key words
muscovy duck reovirus/artificial infection/muscovy duck/liver/proteomics analysis分类
农业科技引用本文复制引用
朱果真,黄梅清,程晓霞,郑敏,陈仕龙,陈少莺,俞伏松..番鸭呼肠孤病毒人工感染雏番鸭肝的蛋白质组学分析[J].畜牧兽医学报,2017,48(10):1949-1957,9.基金项目
福建省自然科学基金(2014J01106) （2014J01106）
福建省重大研发平台(2014N2003-7) （2014N2003-7）
国家自然科学基金(31172334) （31172334）
