畜牧兽医学报2017，Vol.48Issue(10)：1969-1975,7.DOI:10.11843/j.issn.0366-6964.2017.10.020
陕西省犊牛安氏隐孢子虫多位点序列分型研究
Multilocus Sequence Typing of Cryptosporidium andersoni from Calves in Shaanxi Province
摘要
Abstract
To study the multilocus sequence types of Cryptosporidium andersoni from calves in Shaanxi Province,36 fecal samples positive for C.andersoni were collected from 0-6 months calves in four regions of Shaanxi province and examined by the polymerase chain reaction (PCR).Sequence analysis was based on four genetic loci,namely CM-MS1,CM-MS2,CM-MS3 and CM-MS16.The results showed that a total of 15 C.andersoni isolates were successfully genotyped in all four loci and divided into 4 subtypes of MLST,namely A4,A4,A2,A1;A4,A4,A4,A1;A5,A4,A4,A1;A1,A4,A4,A1.Among them,the MLST (A4,A4,A4,A1),as the dominant subtype,was widely distributed in various farms and different breeds of calves.These findings indicated that genetic variation was presented in C.andersoni of calves in Shaanxi province and various MLST subtypes were found in these calves.关键词
安氏隐孢子虫/多位点序列分型/犊牛/陕西Key words
Cryptosporidium andersoni/multilocus sequence typing/calves/Shaanxi Province分类
农业科技引用本文复制引用
任冠静,王雪婷,张会军,宋军科,赵光辉..陕西省犊牛安氏隐孢子虫多位点序列分型研究[J].畜牧兽医学报,2017,48(10):1969-1975,7.基金项目
国家重点研发计划(2017YFD0501300) （2017YFD0501300）
国家自然科学基金面上项目(31572509) （31572509）
陕西省科学技术研究发展规划资助项目(2016NY-113) （2016NY-113）
中国博士后面上项目(2016M592848) （2016M592848）
陕西省博士后科研项目资助特别资助 （）
