畜牧兽医学报2017，Vol.48Issue(10)：1976-1982,7.DOI:10.11843/j.issn.0366-6964.2017.10.021
三株弓形虫对昆明鼠致病性的比较研究
Pathogenicity Comparison of Three Toxoplasma gondii Strains to Kunming Mice
摘要
Abstract
Toxoplasma gondii is an obligate protozoan parasite with only one species,but it has rich genetic diversity with very different virulence.We chose three strains of the only species:Pru,BJ,VEG strains to compare their pathogenicity after intraperitoneal injection in Kunming mouse.We evaluated their virulence by clinical symptoms,serum antibody level,organ distribution of parasites and brain burden.Our results showed that mice symptoms infected with type Ⅱ was more significant than type Ⅲ infected group.Mice specific Toxoplasma antibody in serum was detected from the 4th day in type Ⅱ infection group,and remained increase after that until the 64th day,while type Ⅲ group got the peak earlier at the 32nd day.Interestingly,the three strains showed similar distribution features in mice organs:in early stage (the 1st day),parasites were detected in lung,kidney and muscle,then appeared in heart,lung,muscle and brain in midterm (the 8th day),at last (the 32nd day) brain was the only detected organ.During infection,parasites were not be detected in spleen and liver.Our work successfully built up a Toxoplasma infection mice model of three strains and demonstrated their pathogenicity in detail.In addition,there was a certain similar regularity on mice organ distribution at different time among different Toxoplasma genotypes infection,which means organ tropism along time.Our results give reference for Toxoplasma pathogenicity and establishment of its animal infection model.关键词
弓形虫/致病力/组织分布/脑荷虫量Key words
Toxoplasma gondii/pathogenicity/tissue distribution/brain burden of parasites分类
农业科技引用本文复制引用
李祎,李星,周冰洁,李木子,马磊,刘群,刘晶..三株弓形虫对昆明鼠致病性的比较研究[J].畜牧兽医学报,2017,48(10):1976-1982,7.基金项目
国家重点研发计划(2017YFD0501300) （2017YFD0501300）
北京市自然科学基金(6172023) （6172023）
国家自然科学基金(31372424) （31372424）
