畜牧兽医学报2017，Vol.48Issue(10)：1998-2004,7.DOI:10.11843/j.issn.0366-6964.2017.10.024
新疆库蠓源性盖塔病毒的分离与鉴定
Isolation and Identification of Getah Virus from Culicoides in Xinjiang
摘要
Abstract
In order to learn the situation of parasitic virus in Culicoides in Yuli County,Xinjiang,a total of 18 000 Culicoides were collected from Yuli County and divided into 360 groups,with 50 Culicoides in each group.Then the Culicoides were ground and inoculated into BHK-21,C6/36 and Vero cells,respectively.After 5 generations of blind passage,one group of Culicoides made CPE arise in BHK-21.Then the isolated strain was firstly identified to be RNA viruses by drug sensitivity test (5-floxuridinc).Secondly,the amplified primers were identified by arthropodborne RNA viruses and the result showed that the strain belongs to alphaviruses and is suspected to be Getah viruses.Finally,the isolated strain was determined to be Getah viruses by neutralization test,immunoelectron microscopic observation,PCR amplification of Getah virus C gene-specific primer and other methods.In this study,Getah viruses were isolated from Xinjiang Culicoides for the first time and the virus strain presents a closest genetic relationship (98％) with the 2005 Japanese isolated strain,for which the reason might be the introduction of Japanese breeding sires with the disease or their seminal fluid,suggesting that in the import and export trade activities of animal husbandry,quarantine inspection on Getah virus should be strengthened.关键词
新疆/库蠓/盖塔病毒/鉴定Key words
Xinjiang/Culicoides/Getah virus/identification分类
农业科技引用本文复制引用
刘帅,钟旗,加帕尔·哈斯木,薛新梅,丁梦玥,马晓菁,叶锋,马俊杰,易新萍,谷文喜..新疆库蠓源性盖塔病毒的分离与鉴定[J].畜牧兽医学报,2017,48(10):1998-2004,7.基金项目
公益性行业(农业)科研专项(201303035) （农业）
自治区公益性科研院所基本科研业务经费资助项目(KY2017002) （KY2017002）
