西南林业大学学报2017，Vol.37Issue(5)：22-27,6.DOI:10.11929/j.issn.2095-1914.2017.05.004
辽宁省杨树主栽品种(无性系)AFLP分析
AFLP Analysis of Main Poplar Varieties ( clones) in Liaoning Province
摘要
Abstract
Eight pairs of AFLP primers were screened out and used for the analysis of 28 main poplar varieties in Liaoning Province. The results showed that 983 fragments were generated totally. 972 of which were polymorphic with a polymorphism percentage of 98. 75%. All these date indicated that considerable genetic difference existed a-mong varieties and clones at DNA level. The genetic differences among testing varieties were great, genetic distance among 28 materials tested was 0. 4687-0. 9960. P. × Liaoningensis and P. ×′Liaoyu-3′(0. 4687), P. × canaden-sis ′014′and P. × canadensis ′Guariento′ ( 0. 4755 ) , P. × canadensis ′177′ and P. × canadensis ′zhongliao 1′(0. 4933) had smaller genetic distance, indicated that close genetic relationship. Using UPGMA cluster analysis from AFLP date, similarity coefficient was 0. 3690-0. 6258 and materials tested were classified into 8 categories at similarity coefficient of 0. 533. When the similarity coefficient at 0. 559, 15 varieties of 3-class can be divided into 5 groups. The classification results were basically consistent with growth performance. P. × Liaoningensis and P. ×′Liaoyu-3′had the highest similarity coefficient of 0. 6258. The findings verified that they had the closest genetic re-lationship at molecular level.关键词
杨树/AFLP/遗传距离/相似系数Key words
poplar/AFLP/genetic distance/similarity coefficient分类
农业科技引用本文复制引用
刘巍..辽宁省杨树主栽品种(无性系)AFLP分析[J].西南林业大学学报,2017,37(5):22-27,6.基金项目
辽宁省科技攻关项目 (2011207002) 资助. （2011207002）
