西南林业大学学报2017，Vol.37Issue(5)：152-158,7.DOI:10.11929/j.issn.2095-1914.2017.05.023
乙酰甲胺磷对菠菜内生细菌的影响
Effects of Acephate on Endophytic Bacteria of Spinacia oleracea
摘要
Abstract
Endophytic bacteria of Spinacia oleracea with spraying and without spraying acephate were isolated with classical techniques. The effects of pesticide on the endophytic bacteria were analyzed based on 16S rRNA se-quences. The results showed that spraying the acephate had significantly decreased the number of endophytic bacte-ria in S. oleracea leaves, whereas the number of endophytic bacteria in S. oleracea roots had no significantly differ-ence between spraying acephate and no spraying. 24 endophytic bacterias were isolated from S. oleracea with spra-ying acephate and identified as 6 genera and 7 species, 3 other isolates were possible new species or genus, 2 char-acteristics strains were Pseudomonas monteilii and Arthrobacter arilaitensis. 35 endophytic bacterias were isolated from S. oleracea without spraying acephate and identified as 14 genera and 16 species, 6 other isolates were possible new species or genus. The diversity of culturable endophytic bacteria was reduced with spraying acephate.关键词
乙酰甲胺磷/菠菜/内生细菌/分离/16SrDNAKey words
acephate/Spinacia oleracea/endophytic bacteria/isolation/16S rDNA分类
农业科技引用本文复制引用
刘丽,张俊忠,韩长志,伍建榕,李成云..乙酰甲胺磷对菠菜内生细菌的影响[J].西南林业大学学报,2017,37(5):152-158,7.基金项目
国家重点研发计划项目 (2017YFD0200400) 资助 （2017YFD0200400）
云南省森林灾害预警与控制重点实验室资助 （）
云南省林业一级学科(51600625) 资助. （51600625）
