烟草科技2017，Vol.50Issue(9)：1-6,6.
烟草镉转运基因突变体的鉴定和功能分析
Identification and functional analysis of a tobacco cadmium transporter gene mutant
摘要
Abstract
To obtain Cd-deficiency phenotype in tobacco, the mutant of NtHMA2 gene was screened by TILLING and the contents of Cd and other heavy metals in mutant were detected. The results showed that comparing with the control, the contents of Cd, Zn, Cr, Ni, As and Fe in mutant under Cd stress decreased by 36.84%, 27.92%, 32.48%, 30.18%, 11.33% and 30.51%, respectively. The plant height of mutant was reduced significantly. However, there was no significant difference of the other agronomic traits. The low Cd-content tobacco mutant obtained in this study, hma2-23, could be applied to develop anti-Cd tobacco varieties.关键词
烟草/TILLING技术/NtHMA2基因/突变体/镉含量Key words
Tobacco/TILLING/NtHMA2 gene/Mutant/Cadmium content分类
轻工纺织引用本文复制引用
高玉龙,王丙武,李文正,宋中邦,吴玉萍,李永平..烟草镉转运基因突变体的鉴定和功能分析[J].烟草科技,2017,50(9):1-6,6.基金项目
中国烟草总公司科技重大专项"利用镉转运基因创制云烟87低镉品系"[110201601031(JY-05)] （JY-05）
中国烟草总公司云南省公司科技计划项目"利用镉转运基因创制云烟87低镉品系"(2017YN03). （2017YN03）
