烟草科技2017，Vol.50Issue(9)：30-36,7.
芸薹属植物提取液与异硫氰酸酯类制剂对烟草疫霉菌的抑制作用
Inhibition effects of brassica extracts and isothiocyanates on Phytophthora parasitica var.nicotianae
摘要
Abstract
To obtain effective biological materials against tobacco black shank (Phytophthora parasitica var. nicotianae), the inhibition effects of three brassica extracts and three isothiocyanates on P. parasitica var. nicotianae were detected by mycelium growth rate method and inhibition zone method. The results showed that:Comparing with the control, 1) three brassica extracts (Brassica rapa L., Brassica juncea L. and Brassica campestris L.) at different concentrations significantly inhibited the mycelial growth of P. parasitica var. nicotianae with the EC50values of 11.39, 9.18 and 5.88 mg/mL, respectively. Brassica campestris L. extract had an optimal inhibition effect on the mycelial growth of P. parasitica var. nicotianae. 2) Allylisothiocyanate (AITC) and methylisothiocyanate (MITC) at different concentrations also significantly inhibited the mycelial growth of P. parasitica var. nicotianae with the EC50values of 2.07 and 4.44 mg/mL, respectively. AITC had a better inhibition effect on the mycelial growth of P. parasitica var. nicotianae; however, PITC had no inhibition effect on the mycelial growth of P. parasitica var. nicotianae. In conclusion, three brassica extracts and two isothiocyanates (AITC and MITC) could effectively control P. parasitica var. nicotianae.关键词
烟草黑胫病/烟草疫霉菌/芸薹属/植物提取液/异硫氰酸苯酯/异硫氰酸烯丙酯/甲基异硫氰酸酯/抑制作用Key words
Tobacco black shank/Phytophthora parasitica var. nicotianae/Brassica/Plant extract/Phenylisothiocyanate(PITC)/Allylisothiocyanate(AITC)/Methylisothiocyanate(MITC)/Inhibition effect分类
农业科技引用本文复制引用
李继伟,周俊学,王宇鹏,赵浩宾,赵世民,王慧,刘领..芸薹属植物提取液与异硫氰酸酯类制剂对烟草疫霉菌的抑制作用[J].烟草科技,2017,50(9):30-36,7.基金项目
国家自然科学基金项目"豆科/禾本科间作作物群体对生物炭输入的响应及菌根效应"(31200332) （31200332）
河南省烟草公司项目"基于硫代葡萄糖苷含量的烟草绿肥品种筛选与应用"(HYKJM201307) （HYKJM201307）
河南省高等学校青年骨干教师培养计划项目"生物炭输入对玉米/花生间作种间调控效应及根际微生态机制"(2016GGJS-062). （2016GGJS-062）
