烟草科技2017，Vol.50Issue(10)：16-21,6.
烈性青枯雷尔氏菌噬菌体的分离与生物学特性分析
Isolation and biological characterization of virulent phage infecting Ralstonia solanacearum
摘要
Abstract
To utilize phage therapy in tobacco bacterial wilt controlling, R. solanacearum strain TB15-15 was used as a host bacterium to screen a phage from tobacco soil. Phage titer, multiplicity of infection (MOI), one-step growth curve and phage sensitivity to temperature and pH were measured by a two-layer plating method. The results showed that a virulent phage infecting TB15-15 strain was isolated from soil of a long-term tobacco planting field and named as RS-PII-1. The MOI, phage latent period, burst phase and burst size of RS-PII-1 were 1, 10 min, 100 min, 1 411, respectively. The lytic activity and burst size of RS-PII-1 was the highest at 40 ℃. When temperature was higher than 40 ℃, the activity of RS-PII-1 decreased gradually until it was lost at 60 ℃. Phage had a certain tolerance to pH change, RS-PII-1 showed a good lytic activity in the pH range of 4-8. The lytic activity was reduced when pH <4 or >8, and the phage completely lost lytic activity at pH>11. These results suggested that using RS-PII-1 in an alkaline solution was not suitable.关键词
烟草/青枯病/青枯雷尔氏菌/噬菌体/生物学特性Key words
Tobacco/Bacterial wilt/Ralstonia solanacearum/Bacteriophage/Biological characteristic分类
农业科技引用本文复制引用
苏靖芳,孙剑萍,顾刚,刘俊杰,孙宏伟,郭兆奎,王光华..烈性青枯雷尔氏菌噬菌体的分离与生物学特性分析[J].烟草科技,2017,50(10):16-21,6.基金项目
中国烟草总公司科技重点项目"应用噬菌体防治烟草细菌性病害技术研究"(110201402014). （110201402014）
