烟草科技2017，Vol.50Issue(10)：42-47,6.
DNA甲基转移酶活性对NNKα-亚甲基羟基化代谢的影响
Effects of activity of DNA methyltransferase on α-methylene hydroxylation metabolism of NNK
摘要
Abstract
In order to accurately evaluate the activity of DNA methyltransferase (DNMT) on the amount of O6-methylguanine(O6-mG) produced by α-methylene hydroxylation metabolism of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), ultra-high performance liquid chromatography-high resolution mass spectrometry (UPLC-HRMS) was used to determine the activity of DNMT, the level of DNA methylation, the contents of 4-oxo-4-(3-pyridyl)butyric acid (OPBA), 4-hydroxy-4-(3-pyridyl)butyric acid (HPBA) and O6-mG in the livers and lungs of mice in physiological saline-treated group and decitabine-treated group after 100 mg/kg NNK administration separately. The results showed that: 1) Decitabine inhibited the activity of DNA methyltransferase and decreased the level of DNA methylation. 2) Decitabine did not significantly affect the contents of OPBA and HPBA produced by α-methylene hydroxylation metabolism of NNK. 3) The amount of O6-mG produced through α-methylene hydroxylation metabolism of NNK could be reduced by inhibiting the activity of DNA methyltransferase.关键词
DNA甲基转移酶(DNMT)/DNA甲基化/4-(甲基亚硝氨基)-1-(3-吡啶基)-1-丁酮(NNK)/地西他滨/O6-甲基鸟嘌呤(O6-mG)/超高效液相色谱-高分辨质谱联用(UPLC-HRMS)Key words
DNA methyltransferase (DNMT)/DNA methylation/4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone(NNK)/Decitabine/O6-Methylguanine (O6-mG)/Ultra-high performance liquid chromatography-high resolutionmass spectrometry (UPLC-HRMS)分类
轻工纺织引用本文复制引用
王荣浩,李琛,毛健,刘帅东,王丁众,张启东,霍现宽,张建勋..DNA甲基转移酶活性对NNKα-亚甲基羟基化代谢的影响[J].烟草科技,2017,50(10):42-47,6.基金项目
国家自然科学基金面上项目"改性介孔材料固相萃取/UPLC-MSn方法研究烟草特有亚硝胺在动物体内代谢"(21175153) （21175153）
国家自然科学基金青年基金项目"烟碱在大鼠脑内代谢的活体动态分析及其神经递质化学效应的实时评价"(21307163). （21307163）
