烟草科技2017，Vol.50Issue(10)：55-61,7.
UPLC-MSn法分析烟叶中蔗糖四酯类化合物
Analysis of sucrose tetraesters in tobacco leaves by UPLC-MSn method
摘要
Abstract
A method for analyzing sucrose tetraesters (STEs) in tobacco leaves by ultra-performance liquid chromatography-multi-stage mass spectrometry (UPLC-MSn) was developed. The STEs in tobacco samples were extracted by methanol with ultrasonic-assisted extraction under conditions optimized by orthogonal experimental design. The extracts from tobacco samples were cleaned up and then analyzed qualitatively and quantitatively by UPLC-MSn under selected reaction monitoring (SRM) mode. Five groups of STEs were separated and identified by comparing with standard compounds and analyzing the MSn data of the target objects, and the contents of the five groups of STEs were simultaneously and quantitatively determined by using internal standard curves. The results showed that: 1) The optimum conditions were solid/liquid ratio 1:30 [mass (g):volume (mL)], ultrasonic power 120 W and extraction time 40 min. 2) The standard recoveries of the quantitative analysis method was in the range of 80.67%-89.35%. The relative standard deviations were less than 5% and the correlation coefficients were no less than 0.999 0. The limits of detection (LODs) and quantitation (LOQs) were 14.71-38.42, 49.02-128.05 μg/kg, respectively. This method is suitable for the quantitative analysis of STEs in tobacco leaves with the advantages of simple pretreatment, higher sensitivity and accuracy.关键词
烟叶/超高效液相色谱-多级质谱(UPLC-MSn)/蔗糖四酯Key words
Tobacco leaf/Ultra-performance liquid chromatography-multi-stage mass spectrometry (UPLC-MSn)/Sucrose tetraester分类
轻工纺织引用本文复制引用
贾春晓,李博,魏涛,黄申,毛多斌..UPLC-MSn法分析烟叶中蔗糖四酯类化合物[J].烟草科技,2017,50(10):55-61,7.基金项目
中国烟草总公司卷烟增香保润重大科技专项项目"糖类衍生物香味前体物质的合成、分析及其应用技术研究"[110201101025(BR-07)]. （BR-07）
