烟台大学学报（自然科学与工程版）2017，Vol.30Issue(4)：287-291,5.DOI:10.13951/j.cnki.37-1213/n.2017.04.004
基于线粒体COⅢ基因变异鉴定黄渤海地区海鱼种类
Identification of Marine Fish Species in Huang-Bohai Sea Area Based on Mitochondrial DNA COⅢ Gene Mutation
摘要
Abstract
According to the nucleotide database published in GenBank,a pair of universal primers are designed to amplify a fragment of the mitochondrial COⅢgene in different fish species.The expected fragment of the COⅢgene is amplified from seven important commercial marine fish species in the Yellow Sea and the Bohai Sea,including Acanthogobius hasta,Liza haematocheila,Carassius auratus,Cynoglossus,Thamnaconus modestus,Hexagrammos otakii and Argyrosomus argentatus).The amplified products are sequenced and analyzed with the molecular biology software,Nlal restriction endonuclease is used for digestion,and the specific fragments of the fish species are obtained.The results indicate that polymerase chain reaction-based restriction fragment length polymorphism (PCRRFLP) can effectively distinguish the above seven marine fish species.PCR-RFLP has the advantages of fast and accurate in fish species identification.关键词
通用引物/线粒体COⅢ基因/PCR-RFLP/鱼种鉴定Key words
universal primers/mitochondrial COⅢ gene/PCR-RFLP/species identification分类
生物科学引用本文复制引用
刘军,王秋月,梁衡,李瑶瑶,刘云国,王敏强..基于线粒体COⅢ基因变异鉴定黄渤海地区海鱼种类[J].烟台大学学报（自然科学与工程版）,2017,30(4):287-291,5.基金项目
国家自然科学基金资助项目(11371070). （11371070）
