烟台大学学报（自然科学与工程版）2017，Vol.30Issue(4)：292-300,9.DOI:10.13951/j.cnki.37-1213/n.2017.04.005
基于CODEHOP的欧李钙调蛋白基因片段的克隆
Cloning of Calmodulin Gene Fragment from Cerasus humilis Using CODEHOP-Designed Degenerate Primers
摘要
Abstract
In this study,a pair of degenerate primers,F1:5’-GGCTGACCAACTGACCga (c/t) ga (c/t) ca (a/g) at-3 ' F2:5-CACGTCAGCCTCTCTGatcat (c/t) tc (a/g) tc-3;are designed by CODEHOP.And degenerate primers,N1:5'-AAATGGC(A/C/G) GAT CAGCT(A/C/T) AC-3',N2:5'-CACTT(A/G) GCCATCATGAC (C/T) TT-3 ',are designed by traditional strategy.Two CaM fragments are amplified from seedling of Cerasus humilis,and the calmodulin gene fragment is sequenced the first time.Blast analysis on NCBI,shows that the sequence has high homology with other calmodulin genes.Compared with Prunus mume,Prunus persica and Prunus avium,the homology rates are more than 90％.The results also suggeste that a degenerate primer designed by CODEHOP is credibility and efficient.Successfully cloning the CaM fragment provied basic information for the research of calmoulin and high-calcium characteristic of Cerasus humilis.关键词
欧李/钙调蛋白基因/简并引物/一致简并杂合寡核苷酸引物(CODEHOP)/克隆Key words
Cerasus humilis/calmodulin gene/degenerate primer/CODEHOP(Consensus Degenerate Hybrid Oligonuleotide Primers)/cloning分类
农业科技引用本文复制引用
邬晓勇,孙雁霞,梁勇,陈月星,苟小军..基于CODEHOP的欧李钙调蛋白基因片段的克隆[J].烟台大学学报（自然科学与工程版）,2017,30(4):292-300,9.基金项目
教育部地方高校国家级大学生创新创业训练计划项目(201411079017) （201411079017）
成都大学大学生创新试验计划(CDUCX2015108). （CDUCX2015108）
