烟台大学学报（自然科学与工程版）2017，Vol.30Issue(4)：307-312,6.DOI:10.13951/j.cnki.37-1213/n.2017.04.007
HPLC-MS/MS法测定人参制剂中Rg1、Rb1、Re、Rd的含量
Detection of Ginsenoside Rg1, Rb1, Re, Rd in Preparations Using HPLC-MS/MS
摘要
Abstract
A sensitive and rapid liquid chromatography-tandem mass spectrometry method is developed to determine the content of ginsenoside Rg1,Rb1,Re,Rd in the ginseng preparation.The column of Waters symmetry C18 (150 mm ×2.1 mm,3.5 μm) is employed for separation,the mobile phase is composed of 50％ acatonitril,20％methanol and 0.1％ formic acid,and the flow rate is 0.2 mL/min.The data are acquired by the multiple reactions monitoring in the positive ion mode.In these chromatographic conditions,all the compounds show good linearity.The RSD of precision,repeatability and recovery in experiments are less than 6％.Four ginsenosides separation is achieved with the mobile phase isocratic elution in a total running time of 3.5 min.This method is proven to be specific,accurate,sensitive,and can be successfully applied to determine the contents of four ginsenoside in the ginseng preparations.关键词
液相色谱质谱联用法/多反应监测/人参总皂苷/定量分析Key words
high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS)/multiple reactions monitoring/ginsenosides/quantitative analysis分类
医药卫生引用本文复制引用
姚贞名,庄绪慧,刘文荣,倪莹莹,王文艳..HPLC-MS/MS法测定人参制剂中Rg1、Rb1、Re、Rd的含量[J].烟台大学学报（自然科学与工程版）,2017,30(4):307-312,6.基金项目
国家自然科学基金资助项目(81102880) （81102880）
山东省优秀中青年科学家科研奖励基金项目(BS2011YY062). （BS2011YY062）
