中国临床药学杂志2017，Vol.26Issue(5)：327-330,4.
HPLC法测定哮喘片中麻黄碱的含量
HPLC method for determining the content of ephedrine in asthma tablets
廖刚 1伍玉萍1
作者信息
- 1. 湖南省邵阳市中心医院药学部,邵阳422000
- 折叠
摘要
Abstract
AIM To optimize the chromatographic conditions for using the HPLC method to measure the ephedrine content in asthma tablets.METHODS The HPLC method was used to measure the content.Chromatographic conditions:a Diamonsil C18 chromatographic column (250 mm × 4.6 mm,5 μm),with the methanol-0.5％ triethylamine (2∶ 98) (the pH value adjusted with the phosphoric acid to 2.5) as the mobile phase,and with the flow rate of 1.0 mL · min-1,the column temperature of 30℃ and the test wavelength of 210 nm.The content was measured with the external standard method.RESULTS The ephedrine,as the main active component in asthma tablets,had good baseline separation with other components.The peak area and sampling size of the ephedrine had good linear relationship in the range of 0.051 18-0.451 44 μg (r =0.999 9).The average sample recovery rate was 99.98％ and RSD =0.7％.CONCLUSION The method has the advantages of good linearity,stability,reproducibility and recovery rate,and the result is accurate and reliable,and therefore can be used as a quality control detection method for asthma tablets.关键词
HPLC法/哮喘片/麻黄碱/色谱条件Key words
HPLC/asthma tablet/ephedrine/chromatographic condition引用本文复制引用
廖刚,伍玉萍..HPLC法测定哮喘片中麻黄碱的含量[J].中国临床药学杂志,2017,26(5):327-330,4.
