中国美容医学2017，Vol.26Issue(10)：64-67,4.
具有皮肤美容应用潜能的透皮型弹性蛋白样多肽的制备
Preparation of an Elastin-like Polypeptide Fusion Protein with Transdermal Properties as a Skin Cosmetics Agent
白转丽 1都慧聪 2耿文鑫 2李立文 2舒茂国1
作者信息
- 1. 西安交通大学第一附属医院整形美容·颌面外科 陕西 西安 710061
- 2. 西北大学西部资源生物与现代生物技术教育部重点实验室 陕西 西安 710069
- 折叠
摘要
Abstract
Objective To design and express an elastin-like polypeptide (ELP) fusion protein which can be transcutaneously delivered to skin tissues to explore its potential as a skin cosmetics agent in the future. Methods A protein transduction domain (PTD)-ELP fusion protein with transdermal properties was designed and back-translated with E.coli-preferred codons. And its expression vector was constructed by overlap extension PCR and recursive directional ligation. After transforming the vector into Escherichia coli cells, the PTD-ELP protein was expressed by IPTG induction and the subcellular location and the solubility of the recombinant protein were analyzed. Results A prokaryotic vector containing PTD-ELP-coding cassette was successfully constructed as designed. And the E.coli cells harboring the vector could express the fusion protein after IPTG induction, which reached the highest level at 5h post induction with amount of 23.6% of total bacterial proteins. Further analysis demonstrated that PTD-ELP protein was subcellularly located in cytoplasm as insoluble inclusion bodies. Conclusion The recombinant prokaryotic expression vector was constructed successfully and the PTD-ELP fusion protein was efficiently expressed in Escherichia coli cells. These results laid a foundation for further investigating whether PTD-mediated transdermal delivery of ELP could prevent skin aging and reverse photoaging.关键词
弹性蛋白样多肽/蛋白转导功能域/原核表达/重组蛋白Key words
elastin-like polypeptide/protein transduction domain/prokaryotic expression/fusion protein分类
生物科学引用本文复制引用
白转丽,都慧聪,耿文鑫,李立文,舒茂国..具有皮肤美容应用潜能的透皮型弹性蛋白样多肽的制备[J].中国美容医学,2017,26(10):64-67,4.
