中国麻业科学2017，Vol.39Issue(5)：234-240,7.
一株产耐热甘露聚糖酶细菌的筛选及其酶学性质初探
Screening of a Thermophilic Mannanase Producing Bacterium and Studies on the Enzymatic Properties of Mannanase
摘要
Abstract
A mannanase producing bacterial strain named Mas1 was newly isolated from a hot spring in Jiangxi Province.Mas1 was identified as B.licheniformis by 16SrRNA gene sequence analysis,morphological detection,physiological and biochemical identification.The effect of temperature,pH and metal ions on the activity and stability of crude mannanases from B.licheniformis Mas1 was studied.The results showed that the optimal temperature and pH for the mannanase activity was 60 ℃ and 5.0 respectively;Co2 + and Al3 + were the most effective inhibitor of the enzyme activity,while Mn2 +,K + and Ca2 + could promote the activity of the enzyme,and other ions had no significant effect on the enzyme activity.This strain could be used for the production of mannan-degrading enzymes,and has great potential for applications in textile,food,feed and other fields.关键词
耐热甘露聚糖酶/地衣芽孢杆菌/筛选/酶学性质Key words
thermophilic mannanase/Bacillus licheniformis/screening/enzymatic properties分类
生物科学引用本文复制引用
王瑞君,成莉凤,冯湘沅,段盛文,郑科,刘志远,曾洁,刘正初..一株产耐热甘露聚糖酶细菌的筛选及其酶学性质初探[J].中国麻业科学,2017,39(5):234-240,7.基金项目
中国农业科学院创新工程(ASTIP-IBFC08) （ASTIP-IBFC08）
湖南省自然科学基金(2016jj3126) （2016jj3126）
国家现代农业产业技术体系建设专项项目(CARS-19-E24) （CARS-19-E24）
