中国水产科学2017，Vol.24Issue(5)：1115-1122,8.DOI:10.3724/SP.J.1118.2017.16268
氨和空气暴露对大鳞副泥鳅组织中谷氨酰胺含量的影响
Changes to tissue glutamine content, glutamine synthetase, and glutamate dehydrogenase activities during ammonia and aerial exposure in Chinese loach (Paramisgurnus dabryanus)
摘要
Abstract
Chinese loaches (body weight:18-25 g) were exposed to 30 mmol/L NH4C1 solution and air (exposure time:0,6,12,24,48,72 h) to assess changes in tissue glutamine content,glutamine synthetase (GS) activity,and glutamate dehydrogenase (GDH) activity during ammonia loading and aerial exposure.Glutamine accumulation in liver and muscle,as well as GS activity in the brain,liver,and intestine,were observed with increasing duration of ammonia and aerial exposure.In all analyzed tissue except the liver,GDH activity was significantly affected by ammonia and aerial exposure.Our results suggested that Chinese loaches respond to internal ammonia increase through glutamine accumulation.Glutamine synthetase then stimulates the glutamine formation pathway and converts ammonia into non-toxic glutamine.The marked increase of GDH activity in the intestines demonstrated that intestinal GDH is more important than intestinal GS in the overall defense against ammonia toxicity.Across the exposure period,variation in liver GDH activity was not affected by ammonia and air,nor associated with a concomitant increase in GS activity and glutamine content that probably occurred due to efficient glutamine replenishment via transaminase action.关键词
大鳞副泥鳅/谷氨酰胺/氨暴露/空气暴露Key words
Paramisgurnus dabryanus/glutamine/ammonia exposure/aerial exposure分类
农业科技引用本文复制引用
张云龙,张海龙,王凌宇,顾贝易,樊启学..氨和空气暴露对大鳞副泥鳅组织中谷氨酰胺含量的影响[J].中国水产科学,2017,24(5):1115-1122,8.基金项目
国家科技支撑计划项目(2012BAD25B08,201BAD25B00) （2012BAD25B08,201BAD25B00）
安徽省高等学校自然科学研究重点项目(KJ2017A130). （KJ2017A130）
