中国水稻科学2017，Vol.31Issue(4)：335-344,10.DOI:10.16819/j.1001-7216.2017.7007
水稻抗稻瘟病突变体lmm326的鉴定与调控通路初步分析
Identification of Rice Blast Resistance Mutant lmm326 and Preliminary Analysis of Its Regulatory Pathway
摘要
Abstract
[Objective]Rice lesion mimic mutants often have a certain resistance to rice blast. To understand the genetic mechanisms behind its blast resistance, we identified the rice lesion mimic mutant lmm326 and analyzed its regulatory pathway. [Method] lmm326 was obtained from an EMS-induced Zhonghua 11 mutant bank, and population derived from lmm326/ Zhonghua 11 and lmm326/Dular were used for genetic and fine mapping. [Result]At 5-leaf stage, there were brown lesions on lower leaves of lmm326 initially. Compared with the wild type(WT), photosynthetic pigment contents, net photosynthetic rate, plant height, seed setting rate, tiller number, and 1000-grain weight of lmm326 were significantly reduced. Evans blue and DAB staining assay indicated that leaves of lmm326 accumulated more dead cells and H2O2. Additionally, lmm326 displayed higher resistance to 4 races of rice blast compared with WT. According to genetic analysis, the phenotype of mutant was controlled by a single recessive gene. Here, we employed map-based cloning approach to finely map LMM326 through F2 population derived from a cross between lmm326 and Dular, and the gene was narrowed to a 38-kb region on the long arm of chr. 1, which harbored six ORFs. Sequence analysis revealed that a single base substitution at position C433T in Os01g0919900 CDS resulted in a substitution of leucine for phenylalanine at position 145. Real-time PCR showed that expression levels of defense-related genes were significantly higher in lmm326 than in WT.[Conclusion]These results demonstrated that the target gene is allelic to OsSSI2, and LMM326 is likely to participate in salicylic acid-signaling pathway, and its mutation activates the defense response.关键词
水稻/稻瘟病/防卫反应/类病斑Key words
rice/rice blast/defense response/lesion mimic分类
生物科学引用本文复制引用
徐婷婷,陈代波,占小登,程式华,曹立勇,余宁,张迎信,毕真真,吴玮勋,曹永润,王备芳,张越,轩丹丹..水稻抗稻瘟病突变体lmm326的鉴定与调控通路初步分析[J].中国水稻科学,2017,31(4):335-344,10.基金项目
浙江省自然科学基金资助项目(LY16C130014) （LY16C130014）
转基因重大专项(2016ZX08001-002) （2016ZX08001-002）
国家重点研发计划资助项目(2016YFD0101801). （2016YFD0101801）
