中国水稻科学2017，Vol.31Issue(4)：355-363,9.DOI:10.16819/j.1001-7216.2017.6165
水稻白条纹突变体st13的表型分析及基因定位
Phenotypic Analysis and Gene Mapping of a White Stripe Mutant st13 in Rice
摘要
Abstract
[Objective]Rice leaf color mutation identification and cloning of related genes is helpful to research photosynthesis and further understand the mechanism of chloroplast development and pigment synthesis pathway, providing theoretical basis for the breeding of rice with high photosynthetic efficiency.[Method]A somaclonal mutant (designated as stripe leaf 13, st13) was isolated from tissue-cultured progenies of a japonica rice variety Dongjin. The main agronomic traits of wild type and st13 were determined at maturity, and the pigment contents and ultrastructure of chloroplast were observed at the seedling stage. Dongjin and st13 were reciprocally crossed to observe the phenotype of F1 and genetic analysis was carried out. An F2 population and F2:3 population derived from the cross st13 × Nanjing 11 (an indica rice variety) were used for gene mapping of ST13. Quantitative RT-PCR was carried out to analyze the relative expression of genes associated with chloroplast development and chlorophyll biogenesis in the wild-type Dongjin and st13 mutant.[Result]Compared to the wild type, the plant height, number of effective panicles, panicle length, seed-setting rate, and 1000-grain weight were all significantly reduced in the mutant. In agreement with leaf color, the pigment content of st13 mutant was reduced at the seedling stage but rose to almost the same level as the wild type at the tillering stage. With a transmission electron microscopy, defective chloroplasts with no thylakoid were observed in the st13 mutant. Genetic analysis indicated the mutant phenotype was controlled by a single recessive nuclear gene. ST13 was restricted to an interval between the InDel (insertion/deletion) markers I3-21 and I3-22 on chromosome 3. Six InDel markers were further developed and the ST13 gene was narrowed down to a 94-kb region, containing eight putative open reading frames.[Conclusion]These eight candidate genes encode three putative proteins and five functional proteins respectively, while none of them have been reported in rice. Thus, ST13 was an unreported gene in rice. Relative to wild type, the expression levels of genes associated with chloroplast development, chlorophyll biosynthesis, and photosynthetic system were significantly altered in the mutant. So we hypothesized that ST13 might be the key gene for regulating chloroplast development.关键词
水稻/白条纹突变体/叶绿体/精细定位Key words
rice/white stripe mutant/chloroplast/fine mapping分类
生物科学引用本文复制引用
孙立亭,林添资,王云龙,牛梅,胡婷婷,刘世家,王益华,万建民..水稻白条纹突变体st13的表型分析及基因定位[J].中国水稻科学,2017,31(4):355-363,9.基金项目
国家重点研发项目七大农作物育种专项(2016YFD0100101-08) （2016YFD0100101-08）
国家 863 计划资助项目(2014AA10A603-15) （2014AA10A603-15）
江苏省科技支撑计划资助项目(BE2014394, BE2015363) （BE2014394, BE2015363）
江苏省农业科技自主创新资金资助课题[CX(16)1029]. （16）
