中国水稻科学2017，Vol.31Issue(4)：364-370,7.DOI:10.16819/j.1001-7216.2017.7017
基于重测序的染色体片段代换系定位水稻抽穗期QTL
Mapping of QTLs for Heading Date Using Whole-genome Re-sequenced Chromosome Segment Substitution Lines in Rice
摘要
Abstract
[Objective]Heading date is an important agronomic trait in rice, it is a typical quantitative trait controlled by multiple genes. As novel research material, chromosome segment substitution lines are useful in QTL fine mapping and cloning because of minimizing the interference of genetic background among plants. [Method]In this study, 128 whole-genome re-sequenced chromosome segment substitution lines derived from Nipponbare as donor parent in the background of 9311, were used for mapping QTLs for heading date by combining the sequencing-based Bin-map with multiple linear regression analysis. [Result]Six QTLs for heading date were identified in two environments and two years. qHD2.1 was mapped in the region of 759848 bp on the chromosome 2; qHD2.2 was mapped in the region of 45286 bp on the chromosome 2; qHD 3.1 was mapped in the region of 147931 bp on the chromosome 3; qHD5.1 was mapped in the region of 213351 bp on the chromosome 5; qHD5.2 was mapped in the region of 442305 bp on the chromosome 5;qHD8.1 was mapped in the region of 538176 bp on the chromosome 8. [Conclusion]The results are important for the QTLs cloning and provide a foundation for understanding molecular regulation mechanism of heading date in rice.关键词
水稻/染色体片段代换系/重测序/抽穗期Key words
rice/chromosome segment substitution lines/re-sequenced/heading date分类
生物科学引用本文复制引用
王军,梁国华,朱金燕,陶亚军,周勇,范方军,李文奇,王芳权,仲维功,杨杰..基于重测序的染色体片段代换系定位水稻抽穗期QTL[J].中国水稻科学,2017,31(4):364-370,7.基金项目
国家 973 计划资助项目(2013CBA01405) （2013CBA01405）
公益性行业科研专项(201303102) （201303102）
江苏省现代农业重点研发项目(BE2015355,BE2015341) （BE2015355,BE2015341）
扬州市农业前瞻性研究资助项目(YZ2014165). （YZ2014165）
