中国水稻科学2017，Vol.31Issue(4)：371-378,8.DOI:10.16819/j.1001-7216.2017.7020
聚合水稻温敏核不育基因和反温敏核不育基因创制永久核不育系
Development of Permanent Genic Male Sterile Line by Pyramiding Thermo-sensitive Genic Male Sterile Genes and Reverse Temperature Induced Genic Male Sterile Genes in Rice (Oryza sativa L.)
摘要
Abstract
[Objective]The security problem of seed production has seriously hampered the steadily and sustainable development of two-line hybrid rice.[Method]Aizi S (thermos-sensitive genic male sterile, TSGMS) and Aiyan s (reverse temperature sensitive genic male sterile line, RTSGMS) were screened as the original parents to develop permanent genic sterile line (PGSL) according to the F1 fertility of crosses among 16 (light-) TSGMS and 4 RTSGMS. The fertility genes were introduced into the near isogenic lines Tianfeng S and Tianfeng s by backcrossing Aizi S and Aiyan s with the bridge parent Tianfeng B, respectively. Then, Tianfeng Ss, the PGSL, was observed from the cross between Tianfeng S and Tianfeng s. The fertility, restoration, competitive advantage of yield-related traits and reproductive characteristics of Tianfeng Ss were investigated.[Result] Tianfeng Ss was completely sterile under natural long day with high temperature and short day with low temperature. And no obvious difference was noted among the F1 crosses from Tianfeng Ss and its three parents and five restorers in spikelet fertility percentage. [Conclusion]The results showed that the newly developed PGSL might solve the security problem of seed production in two-line hybrid rice fundamentally.关键词
水稻/温敏核不育/反温敏核不育/永久核不育系Key words
rice/thermo-sensitive genic male/reverse thermo-sensitive genic male/permanent genic sterile line分类
生物科学引用本文复制引用
江建华,倪金龙,吴爽,王德正..聚合水稻温敏核不育基因和反温敏核不育基因创制永久核不育系[J].中国水稻科学,2017,31(4):371-378,8.基金项目
安徽省科技计划资助项目(1501031096) （1501031096）
国家重点研发计划资助项目(2016YFD0101105). （2016YFD0101105）
