中国生物防治学报2017，Vol.33Issue(5)：612-622,11.DOI:10.16409/j.cnki.2095-039x.2017.05.006
入侵种西花蓟马与本地近缘种花蓟马的双基因鉴定
Identification of Invasive Species Frankliniella occidentalis and Native Species F.intonsa Based on Double Gene Markers
摘要
Abstract
Rapid and accurate species identification is crucial in natural enemy screening and management of pest insects by biological control.Two closely related thrips species,Frankliniella occidentalis (an invasive thrips species) and F.intonsa (a native thrips species),always occur simultaneously at the same district,during the same period or even on the same host plant.Since both thrips species are small in size and morphologically similar,it is very difficult to distinguish between the two of them rapidly.In the present study,the mitochondriat cytochrome c oxidase subunit Ⅰ (COI) and internal transcribed spacer 2 (ITS2) genes were used to identify F.occidentalis and F.intonsa,collected from 10 different districts of seven provinces in China.The results based on COI and ITS2 genes indicated that the genetic distance between the two thrips species was 0.221 and 0.113,respectively,which were 22.1 and 18.8 times as high as the corresponding values within species,and no overlap was detected between interand intra-species genetic distances.The identification based on phylogenetic trees was consistent with those based on morphological characteristics for F.occidentalis and F.intonsa,indicating that both COI and ITS2 genes can be used to identify closely related species effectively.Moreover,when the COI gene sequence was analyzed,all the haplotypes from F.occidentalis assembled into two clades corresponding to glasshouse strain and lupin strain of this thrips.The results can benefit identification of closely related species accurately and rapidly,as well as screening and exploiting specialist natural enemies and evaluating their control efficacy.关键词
花蓟马/西花蓟马/近缘种/双基因鉴定/遗传距离/系统发育分析Key words
Frankliniella intonsa/F.occidentalis/closely related species/identification based on double gene sequences/genetic distance/phylogenetic analysis分类
农业科技引用本文复制引用
田虎,张蓉,张金良,王玉生,万方浩,张桂芬..入侵种西花蓟马与本地近缘种花蓟马的双基因鉴定[J].中国生物防治学报,2017,33(5):612-622,11.基金项目
国家重点研发计划(2017YFC1201200,2016YFC1201200) （2017YFC1201200,2016YFC1201200）
国家自然科学基金(31572067) （31572067）
农业部“948”计划(2016-X48) （2016-X48）
中国农业科学院科技创新工程项目 （）
