中国生物防治学报2017，Vol.33Issue(5)：623-630,8.DOI:10.16409/j.cnki.2095-039x.2017.05.007
黏虫热休克蛋白Hsp90基因的克隆及不同温度对其诱导反应
cDNA Cloning and Induction of Heat Shock Protein Hsp90 from Mythimna separata
摘要
Abstract
Heat shock proteins are key elements of the organism's adaptive response to adverse environments.Hsp90 has an important place in the Hsp superfamily.Full length of Hsp90 cDNA sequence was obtained from Mythimna separata by using high-throughput sequencing.Hsp90 mRNA transcripts in M.separata were examined at various developmental stages and in different tissues based on real-time PCR.Hsp90 mRNA expression levels were also examined to different temperature treatments.The results showed that the cDNA sequence of Hisp90 in M.separata is 2422 bp and encodes 717 amino acids.The molecular mass is about 82.576 ku,and isoelectric point is about 4.98.The amino acid sequence includes signature sequences of Hsp90.Sequence alignment showed that MsHsp90 shared more than 98％ identity with Hsp90 sequences reported in Spodoptera exigua,Spodoptera litura and Heliothis viriplaca.The cDNA sequence was designated as MsHsp90 (GenBank accession No.MF773751).The relative expression of MsHsp90 in the 2nd instar larvae and hindgut was higher than that in other developmental stages and tissues.The relative expression level of MsHsp90 in M.separata varies in different temperature stress and time periods.Based on the analysis above,the relative expression level of MsHsp90 in different tissues,at different developmental stages and in different temperature induction indicated that MsHsp90 may play different roles in M.separata.关键词
黏虫/热休克蛋白Hsp90/温度胁迫Key words
Mythimna separata/heat shock protein 90/temperature induction分类
农业科技引用本文复制引用
杨静,Nasir Ali Baloch,樊东..黏虫热休克蛋白Hsp90基因的克隆及不同温度对其诱导反应[J].中国生物防治学报,2017,33(5):623-630,8.基金项目
黑龙江省自然科学基金(C201415) （C201415）
