中国生物防治学报2017，Vol.33Issue(5)：650-657,8.DOI:10.16409/j.cnki.2095-039x.2017.05.011
枯草芽胞杆菌VD18R19在胡椒上的定殖动态及促生作用和对胡椒瘟病的防治效果
Colonization Dynamics and Growth Promotion Effect of Bacillus subtilis VD18R19 in Black Pepper Plant and Its Biological Control Effects on Phytophthora Rot Disease
摘要
Abstract
Colonization is an important factor for application of beneficial Bacillus genus bacteria,however,colonization of B.subtilis in black pepper has not been reported yet.In this study,B.subtilis VD 18R19 was applied to black pepper plants to investigate the colonization dynamics,growth promotion effect and the biological control effects on Phytophthora rot disease.To evaluate colonization ability,strain VD18R19 was labeled with green fluorescent protein (GFP).Green fluorescent light could be observed from the GFP-tagged strain under microscope,and the genetic stability was 85.33％ after 120 generations.Distinct colonization tendencies were exhibited when treated by leaf spraying and root irrigating.At rhizosphere,increased colonization was initially observed over time to the 9th day,followed by decreased levels to the 21 st day that were subsequently maintained at a stable level for the duration of the experiment.The population densities of VD18R19 were at 106 CFU/g level through the experiment.At leaf surface,the colonization was decreased quickly,and few stains could be detected after 21 days.In the absence of pathogen,the VD 18R19 treatments could significantly increase the plant height,leaf number and fresh weight,with the promotion rates of 61.71％,36.18％ and 106.43％,respectively.When challenging the pathogen Phytophthora capsici,plants treated by VD18R19 showed much lower disease incidence and less severe disease symptoms,with the control efficiency of 76.86％.The results are useful for development and application of B.subtilis in the future.关键词
枯草芽胞杆菌/胡椒瘟病/定殖/生物防治/促生长Key words
Bacillus subtilis/black pepper Phytophthora rot/colonization/biocontrol/growth promotion分类
农业科技引用本文复制引用
高圣风,刘爱勤,桑利伟,苟亚峰,孙世伟,王政,孟倩倩..枯草芽胞杆菌VD18R19在胡椒上的定殖动态及促生作用和对胡椒瘟病的防治效果[J].中国生物防治学报,2017,33(5):650-657,8.基金项目
国家自然科学基金(31501690) （31501690）
中国热带农业科学院基本科研业务费专项资金(1630042017022) （1630042017022）
