中国生物防治学报2017，Vol.33Issue(5)：658-666,9.DOI:10.16409/j.cnki.2095-039x.2017.05.012
荧光假单胞菌PEF-5#18防控番茄枯萎病的定殖机理
Colonization of Pseudomonas fluorescens PEF-5#18 to Control Fusarium Wilt Disease on Tomato
摘要
Abstract
In order to study the colonization in rhizosphere soil as well as root-stem inside,plasmid pEGFP20 expressing green fluorescens protien was transferred into wild type Pseudomonasfluorescens PEF-5#18 with great effect against Fusarium wilt disease on tomato by electrophoretic transfer method.The results indicated that both wild type and marked P.fluorescens PEF-5#18 bio-control treatments could remarkably control Fusarium wilt disease on tomato with the efficiency of 76.92％ and increase plant fresh (194.10％) or dry weight (564.71％) after 25 days,if compared with Forl positive treatment.Inoculated P fluorescens PEF-5#18 could colonize in rhizosphere soil and extend into tomato root-stem inside,and the distribution amounts ranked as:rhizosphere soil (1.05 × 106 CFU/g) ＞ root (6.75 × 104 CFU/g) ＞ stem (1.25 × 102 CFU/g).Compared with pathogen positive control,the pathogen population was remarkably reduced in rhizosphere soil and root-stem inside,the cultivable bacteria population (increased 8.42％ inside root and 14.78％ inside stem) and pH value (increased 1.61％) were remarkably increased.Confocal microscopy observation demonstrated that P.fluorescens PEF-5#18 existed on root surface,root xylem,root endodermis cells,root intercellular space,stem vascular tissue cells and the cells around.关键词
荧光假单胞菌/番茄枯萎病/标记/定殖Key words
Pseudomonas fluorescens/tomato wilt/marker/colonization.分类
农业科技引用本文复制引用
张亮,盛浩,袁红,赵兰凤,李华兴..荧光假单胞菌PEF-5#18防控番茄枯萎病的定殖机理[J].中国生物防治学报,2017,33(5):658-666,9.基金项目
广东省教育部产学研结合项目(2009B090300330) （2009B090300330）
