中国生物防治学报2017，Vol.33Issue(5)：667-674,8.DOI:10.16409/j.cnki.2095-039x.2017.05.013
拮抗灰霉菌的海洋细菌甲基营养型芽胞杆菌的筛选、鉴定及其抑菌活性物质的研究
Screening, Identification of Antagonistic Ocean Bacteria Bacillus methylotrophicus and Its Antimicrobial Substances to Botrytis cinerea pres.
摘要
Abstract
464 strains of ocean bacteria were isolated from 65 samples of sea water and sediment from Bohai using ZoBell's 2216E medium.Through plate confrontation method,47 strains of marine bacteria with strong antagonism against Botrytis cinerea were obtained.Strain BH21 with high salt and methanol tolerance had the strongest antifungal effect and the inhibition zone diameter was 11.8 mm±0.3 mm.Strain BH21 was identified as Bacillus methylotrophicus by morphological,physiological,biochemical and molecular methods.Using the specific primers of lipopeptide gene,the related genes in strain BH21 were amplified and 7 lipopeptide antibiotics synthetic genes including ItuA,ituB,ituC,ituD,fend,srfAB andyndJgenes were identified in the strain.Crude extract of lipopeptide was obtained by the hydrochloric acid precipitation and methanol extraction.Oxford cup analysis demonstrated that the crude extract of lipopeptide could inhibit the mycelial growth ofB.cinerea,with the inhibition rate of 73.9％.关键词
海洋细菌/灰霉菌/甲基营养型芽胞杆菌/脂肽类抗生物质/拮抗菌Key words
Marine bacteria/Botrytis cinerea/Bacillus methylotrophicus/lipopeptide antifungal substance/antagonistic microbe分类
农业科技引用本文复制引用
魏新燕,黄媛媛,黄亚丽,杜克久..拮抗灰霉菌的海洋细菌甲基营养型芽胞杆菌的筛选、鉴定及其抑菌活性物质的研究[J].中国生物防治学报,2017,33(5):667-674,8.基金项目
河北省应用基础研究计划重点基础研究项目(15962904D) （15962904D）
国家重点研发计划课题(2016YFC0501303). （2016YFC0501303）
