中国生物防治学报2017，Vol.33Issue(5)：675-680,6.DOI:10.16409/j.cnki.2095-039x.2017.05.014
土壤因子对海洋多粘类芽胞杆菌L1-9在黄瓜根表土壤定殖的影响
Effects of Soil Factors on the Colonization of Marine Biocontrol Bacterium Paenibacillus polymyxa L1-9 in Cucumber Root Surface Soil
摘要
Abstract
In this study,the effects of soil organic matter contents,temperature and soil pH value on colonization of marine biocontrol bacterium of L1-9 in root surface soil were investigated.16S rDNA gene fragments of the strain in the root surface soil were quantified using quantitative real-time PCR method.The results showed that soil organic matter content,temperature and pH exhibited significant impacts on colonization of the strain L1-9 in root surface soil (P ＜ 0.01).The propagation of the strain L1-9 in root surface soil was faster under the temperature of 25 ℃,which was 5.79× 108 copies per gram dry soil,and 4.3 times of that under 15 ℃.Addition of organic matter (20-40 g/kg) increased the population density of the strain L1-9.The maximum colonization level of the strain L1-9 in the root surface soil was achieved in pH 7.This study laid a foundation for the development and application of the marine biocontrol bacterium L1-9 against soilborne diseases of cucumber.关键词
多粘类芽胞杆菌/根表土壤/定殖/实时荧光定量PCRKey words
Paenibacillus polymyxa/root surface soil/colonization/quantitative real-time PCR分类
农业科技引用本文复制引用
王淑芳,汪晶晶,曹雪梅,李欢,王琦,王军强,马桂珍,暴增海..土壤因子对海洋多粘类芽胞杆菌L1-9在黄瓜根表土壤定殖的影响[J].中国生物防治学报,2017,33(5):675-680,6.基金项目
江苏省自然科学基金(BK20141248) （BK20141248）
连云港市科技局现代农业项目(NYYQ1619,CN1514) （NYYQ1619,CN1514）
淮海工学院青年基金项目(Z2014005) （Z2014005）
