中国生物防治学报2017，Vol.33Issue(5)：692-698,7.DOI:10.16409/j.cnki.2095-039x.2017.05.017
列当生防菌层出镰刀菌Br-1发酵条件的优化及田间防治效果
Optimization of Fermentation Medium Components and Conditions of Fusarium proliferatum Br-1 and Its Field Control Efficacy
摘要
Abstract
Biological control agent Br-1 was isolated from diseased broomrape and identified as Fusarium proliferatum.In order to increase the sporulation of strain Br-1,the medium components and fermentation conditions were optimized by single factor and orthogonal design.The results indicated that optimized medium components of strain Br-1 were glucose 1％,NH4NO3 0.2％,KH2PO4 0.3％,MgSO4·7H2O 0.1％.Optimum fermentation conditions of strain Br-1 were pH 5.0,medium capacity 50 mL/250 mL,temperature 24 ℃,rotating speed 190 r/min,fermentation time 6 d,with sporulation of Br-1 increased by 257.5 times in comparison with that under unoptimized conditions.Field trails showed that control efficacy of Br-1 on Orobanche cernua Loefling was up to 67.2％.关键词
层出镰刀菌/发酵优化/培养基/防治效果Key words
Fusarium proliferatum/fermentation condition/medium/biological control分类
农业科技引用本文复制引用
王亚娇,纪莉景,栗秋生,肖颖,王连生,孔令晓..列当生防菌层出镰刀菌Br-1发酵条件的优化及田间防治效果[J].中国生物防治学报,2017,33(5):692-698,7.基金项目
河北省财政项目(F13R10001) （F13R10001）
河北省财政基本科研业务费项目(494-0401-JBN-6440) （494-0401-JBN-6440）
