中国实用口腔科杂志2017，Vol.10Issue(10)：605-608,4.DOI:10.19538/j.kq.2017.10.007
新型钛铜合金对牙龈卟啉单胞菌超微结构及基因组DNA影响研究
Experimental study on the effect of a new titanium-copper alloy on the ultrastructure and genomic DNA of Porphyromonas gingivalis
摘要
Abstract
Objective To study the effect of a new titanium-copper alloy on the ultrastructure and genomic DNA of Porphyromonas gingivalis (P.g).Methods The titanium-copper alloy with copper content of 2％,5％ and 10％ were prepared and named Ti-2％Cu,Ti-5％Cu and Ti-10％Cu,respectively.The commercial pure titanium was used as the control,named cp-Ti.P.g were cultured on the surface of these materials and placed on the cell culture plate.The transmission electron microscope was used to scan the ultrastructure,and agarose gel electrophoresis was used to test the genomic DNA of P.g.Results (1) Under transmission electron microscope:the number of damaged P.g increased with the increase of copper content;the cytomembrane,cytoderm and cytoplasma of P.g in cp-Ti group were intact;the cytoderm and cytomembrane of P.g in Ti-2％Cu,Ti-5％Cu and Ti-10 ％Cu were intact,blured and ruptured,and black precipitate and vacuole appeared in the cytoplasma.(2)The agarose gel electrophoresis showed:there was no difference in the amount of genomic DNA in control or experimental groups after being contacted by new titanium-copper alloy of P.g for 24h.Conclusion Cupper ions in new titanium-copper alloy can damage the ultrastructure of P.g,but cann't degrade the genomic DNA of it in short time.关键词
钛铜合金/抗菌机制/牙龈卟啉单胞菌/透射电镜/琼脂糖凝胶电泳Key words
titanium-copper alloy/antibacterial mechanism/Porphyromonas gingivalis/transmission electron microscope/agarose gel electrophoresis分类
医药卫生引用本文复制引用
张燕婷,谢艳婷,顾舒扬,何晶,江鹭鹭,张翀,邓春富,张二林,赵宝红..新型钛铜合金对牙龈卟啉单胞菌超微结构及基因组DNA影响研究[J].中国实用口腔科杂志,2017,10(10):605-608,4.基金项目
辽宁省自然科学基金(20170541059) （20170541059）
沈阳市科技计划项目(F11-264-1-25) （F11-264-1-25）
辽宁省科技计划项目(2013225049) （2013225049）
