中国畜牧兽医2017，Vol.45Issue(10)：2837-2844,8.DOI:10.16431/j.cnki.1671-7236.2017.10.002
牛副流感3型病毒Nano-PCR、LAMP方法的建立及初步应用
Establishment and Preliminary Application of Nano-PCR and LAMP Methods for Bovine Parainfluenza Type-3 Virus
摘要
Abstract
This study was aimed to establish Nano-PCR and LAMP which were new rapid type of molecular detection technologies of bovine parainfluenza type-3 virus (BPIV3).The comparative specificity and sensitivity of BPIV3 Nano-PCR and LAMP PCR were tested,and the assay was applied to detect 10 clinical samples.The specificity and sensitivity tests showed that Nano PCR and LAMP were only sensitive to BPIV3,without cross reaction to other viruses,such as bovine respiratory syncytial virus,infectious bovine rhinotracheitis virus and bovine viral diarrhea virus.In the sensitivity test,Nano-PCR and LAMP showed 10 times sensitivity than that of the traditional PCR technology,with the minimum detection of 4.16 × 102 copies/μL.Clinical test results showed that the coincidence rate of Nano-PCR and LAMP could reach to 100％,and the positive detection rate was much higher than that of normal PCR.Therefore,the Nano-PCR and LAMP methods established in this study provided a faster,sensitive and reliable tool for the clinical diagnosis of BPIV3.关键词
牛副流感3型病毒(BPIV3)/Nano-PCR/LAMPKey words
bovine parainfluenza type-3 virus (BPIV3)/Nano-PCR/LAMP分类
农业科技引用本文复制引用
李健友,李家伟,王超,郭利,何洪彬..牛副流感3型病毒Nano-PCR、LAMP方法的建立及初步应用[J].中国畜牧兽医,2017,45(10):2837-2844,8.基金项目
国家重点研发计划:畜禽重大疫病防控于高效安全养殖综合技术研发(2016YFD0500907) （2016YFD0500907）
吉林省科技厅重点科技成果转化项目(20150307024NY) （20150307024NY）
吉林省科技厅产业技术创新战略联盟项目(20170309002NY) （20170309002NY）
