中国畜牧兽医2017，Vol.45Issue(10)：2845-2850,6.DOI:10.16431/j.cnki.1671-7236.2017.10.003
STC-1基因在大、小体型猪中表达与分布的差异分析
Expression and Distribution Difference Analysis of STC-1 Gene in Big and Small Body Shape Pigs
摘要
Abstract
The heart,liver,spleen,lung,kidney,skull,skeletal muscle were collected from 40-day-old Bama Mini pig and Large White pig,stanniocalcin 1(STC-1) gene mRNA and STC-1 protein were detected with Real-time PCR and Western blotting methods,respectively.The Real-time PCR results showed that the expression level of STC-1 gene mRNA was higher in lung and kidney,but was the lowest in skeletal muscle of Bama Mini pig and Large White pig.Except for heart and skeletal muscle tissues,the expression level of STC-1 gene mRNA in other tissues of Bama Mini pig was significantly higher than Large White pig (P＜0.05).The Western blotting results showed that the STC-1 protein distribution were the highest in liver and spleen of Bama Mini pig and Large White pig,respectively,and the difference was significant (P＜0.05).The STC-1 protein expression in lung,liver,skeletal muscle and heart of Bama Mini pig were extremely significantly higher than Large White pig (P＜0.01),and the STC-1 protein expression in kidney and spleen of Bama Mini pig were extremely significantly lower than Large White pig (P＜ 0.01).STC-1 gene mRNA and protein were firstly detected in different tissues from big and small body shape pigs,this might have a relationship with various external environments stress and development.关键词
猪/体型/斯钙素-1(STC-1)基因/表达与分布Key words
pig/body shape/stanniocalcin 1 (STC-1) gene/expression and distribution分类
生物科学引用本文复制引用
杨开典,齐传翔,祁钰钰,鞠辉明..STC-1基因在大、小体型猪中表达与分布的差异分析[J].中国畜牧兽医,2017,45(10):2845-2850,6.基金项目
江苏高校优势学科建设工程资助项目(2015) （2015）
国家自然科学基金项目(31272405) （31272405）
江苏高校品牌专业建设工程资助项目(TAAP,2015-2018) （TAAP,2015-2018）
