中国畜牧兽医2017，Vol.45Issue(10)：2878-2885,8.DOI:10.16431/j.cnki.1671-7236.2017.10.008
牛源产志贺毒素大肠杆菌分离株的毒力基因分布和遗传进化分析
Study on the Virulence Gene Distribution and Genetic Evolution of Cattle Shiga Toxin-producing Escherichia coli Isolates
摘要
Abstract
This study was aimed to understand the relationship of virulence gene distribution and genetic evolution between cattle originated Shiga toxin-producing Escherichia coli (STEC) and human originated enterohaemorrhagic Escherichia coli (EHEC) O157.This experiment collected 18 strains STEC in a dairy farm from Jiangsu province and 9 STEC reference strains (human,sheep,swine and avian),according to the method of U.S.Centers for Disease Prevention and Control Center (PulseNet),using the Xba Ⅰ enzyme digestion and pulsed field gel electrophoresis (PFGE) analysis,virulence genes were detected in some STEC isolates.The virulence gene distribution of O157 from different origin was remarkably different.The cattle originated STEC O157 and the human originated EHEC O157 ∶ H7 (EDL933W) had the most similar virulence gene distribution.In contrast,virulence genes were lack in cattle STEC O18 and O26,even though the cattle STEC O18 and O26 had the similar genotype as human EHEC O157 ∶ H7 (EDL933W).PFGE of Xba Ⅰ digested chromosomal DNA from 27 isolates of STEC exhibited 22 profiles.In general,the Dice coefficients of different originated STEC ranged from 72％ to 100％.Cattle STEC O157 had a high similarity with two strains of human originated EHEC O157,while a low similarity was demonstrated between cattle STEC O157 and STEC O157 of swine and avian.The Dice coefficients of the cattle STEC O157 and the two strains of human EHEC O157 ranged from 83％ to 95％.The Dice coefficients of cattle STEC O26 (Ⅶ,Ⅶ) and the two strains of human EHEC O157 were more than 82％.Therefore,it was concluded that the cattle STEC O157 and human EHEC O157 had a closer relationship in terms of virulence gene distribution and in genetic evolution.关键词
产志贺毒素大肠杆菌(STEC)/肠出血性大肠杆菌/毒力基因分布/脉冲场凝胶电泳/遗传进化Key words
Shiga toxin-producing Escherichia coli (STEC)/enterohaemorrhagic Escherichia coli(EHEC)/virulence gene distribution/pulsed field gel electrophoresis/gene evolutionn分类
农业科技引用本文复制引用
薛涛,李丽,高清清,陈先亮,李甜甜,曲信芹,高崧..牛源产志贺毒素大肠杆菌分离株的毒力基因分布和遗传进化分析[J].中国畜牧兽医,2017,45(10):2878-2885,8.基金项目
国家自然科学基金(30972196、30771460、30471281) （30972196、30771460、30471281）
