中国畜牧兽医2017，Vol.45Issue(10)：2897-2907,11.DOI:10.16431/j.cnki.1671-7236.2017.10.010
动物益生菌短短芽胞杆菌FJAT-1501-BPA制剂的制备及其益生特性评价
Preparation of Brevibacillus brevis FJAT-1501-BPA and Its Evaluation as Probiotic
摘要
Abstract
The composition of colloidal suspension agent of Brevibacillus brevis FJAT-1501-BPA was optimized to obtain the stable long-term storage agent,at the same time,the evaluation of this strain as a probiotic strain was conducted in this paper.The effects of different stabilizers,pH,NaCl and preservative concentrations on the preservation characteristics of this strain were studied.And spore yield,heat resistance,antibiotic sensitivity,artificial gastrointestinal tolerance,bile salt tolerance and other biological characteristics of Brevibacillus brevis FJAT-1501-BPA as a probiotic strain were evaluated.The results showed that the colloidal suspension agent of Brevi bacillus brevis FJAT-1501-BPA was composed of 4％ NaCl,0.1‰ calcium citrate,0.1‰ sodium diacetate,0.1‰ potassium sorbate and 2.0％ xanthan gum with pH 7.Brevibacillus brevis FJAT-1501-BPA could inhibit the growth of Salmonella,Escherichia coli and Staphylococcus aureus.It had good tolerance to artificial gastric and intestinal juice,0.3％ pig bile salt,high spore production rate and strong heat resistance,and it could be used in conjunction with streptomycin and ampicillin.Therefore,Brevibacillus brevis FJAT-1501-BPA could be used as a probiotic strain in livestock production.关键词
短短芽胞杆菌/人工胃液/人工肠液/菌剂/抗生素Key words
Brevibacillus brevis/artificial gastric juice/artificial intestinal fluid/microbial agent/antibiotic分类
农业科技引用本文复制引用
车建美,刘波,刘国红,陈倩倩,葛慈斌,蓝江林,叶少文..动物益生菌短短芽胞杆菌FJAT-1501-BPA制剂的制备及其益生特性评价[J].中国畜牧兽医,2017,45(10):2897-2907,11.基金项目
福建省自然科学基金项目(2014J06012) （2014J06012）
农业部公益性行业(农业)科研专项(201303094) （农业）
福建省农业科学院青年英才计划项目(YC2015-7) （YC2015-7）
福建省属公益性项目(2015R1101012-9) （2015R1101012-9）
