中国畜牧兽医2017,Vol.45Issue(10):2957-2964,8.DOI:10.16431/j.cnki.1671-7236.2017.10.018
MYNN基因在猪不同组织及肌肉中的表达规律
The Expression Characteristics of MYNN Gene in Different Tissues and Muscles of Pig
摘要
Abstract
This study was aimed to investigate the expression characteristics of myoneurin (MYNN) gene in different tissues and its developmental expression in muscles (longissimus dorsi,biceps femoris and psoas major),cerebellum,liver,pancreas,kidney,stomach,spleen and lung tissues of pigs.The expression characteristics of MYNN mRNA in 11 different tissues including heart,liver,spleen,lung,kidney,cerebellum,small intestine,pancreas,stomach,biceps femoris and fat of Large White pigs and Mashen pigs at the age of 90 days and the developmental expression patterns in muscle,cerebellum,liver,pancreas,kidney,stomach,spleen at three strages (1,90,180 days) of Large White pigs and Mashen pigs were studied by Real-time PCR.The results showed that MYNN was widely expressed in various tissues of pigs,and there was significant difference among the tissues(P<0.05;P<0.01);The expression of MYNN in muscle,liver,pancreas,cerebellum,kidney,stomach,spleen,lung tissues were significant difference at three development stages of Large White pigs and Mashen pigs(P<0.05;P<0.01),and also had a specific rule,which indicated that it may play an important role in these pig tissues.The expression of MYNN gene could related to the tissue,age and the genetic background of breeds.The results of this study provided a better understanding of the biological functions of pig MYNN.Further studies are required to determine its molecular mechanisms,especially in the regulation of skeletal muscle development.关键词
猪/MYNN基因/表达谱/发育性表达Key words
pig/MYNN gene/expression characteristic/developmental expression分类
生物科学引用本文复制引用
李萌,郭晓红,靳玉舒,张旗,成志敏,张宁芳,乐宝玉,高鹏飞,曹果清,李步高..MYNN基因在猪不同组织及肌肉中的表达规律[J].中国畜牧兽医,2017,45(10):2957-2964,8.基金项目
山西省科技创新重点团队项目(201605D131045-24) (201605D131045-24)
三晋学者项目(2016) (2016)
山西省农业科技攻关项目(20140311020-5) (20140311020-5)