中国畜牧兽医2017，Vol.45Issue(10)：2987-2993,7.DOI:10.16431/j.cnki.1671-7236.2017.10.022
新疆巴什拜羊肌肉生长抑制素基因DNA甲基化与mRNA表达量分析
Analysis of DNA Methylation and mRNA Expression Level of MSTN Gene in Xinjiang Bashiby Sheep
摘要
Abstract
In order to investigate DNA methylation and expression levels of myostatin (MSTN)gene in mscule and fat,5 months of age of Bashiby sheep were selected,the promoter region and exon 1 methylation levels of MSTN gene was analyzed using bisulfite sequencing PCR (BSP).Real-time PCR was used to detect the expression level of MSTN gene in biceps femoris,femoral triceps,semitendinosus,semimembranosus,longissimus dorsi muscle and tail fat of Bashiby sheep.The results showed that the methylation probability of muscle was higher than fat,the methylation probability of biceps femoris,femoral triceps,semitendinosus,semimembranosus,longissimus dorsi muscle and tail fat were 74.2％,74.2％,83.2％,83.7％,82.1％ and 25.3％,respectively.The expression levels of MSTN gene in biceps femoris,femoral triceps,semitendinosus,semimembranosus,longissimus dorsi muscle were significantly lower than tail fat in Bashiby sheep (P＜0.05),but there were no significant difference among biceps femoris,femoral triceps,semitendinosus,semimembranosus and longissimus dorsi muscle (P＞ 0.05).The DNA methylation was negatively correlated with the expression levels in muscle and fat of Bashiby sheep (r=-0.886,P＜0.05).关键词
巴什拜羊/MSTN基因/DNA甲基化Key words
Bashiby sheep/MSTN gene/DNA methylation分类
农业科技引用本文复制引用
姚力丹,祖玲玲,马晓燕,依明·苏来曼,决肯·阿尼瓦什..新疆巴什拜羊肌肉生长抑制素基因DNA甲基化与mRNA表达量分析[J].中国畜牧兽医,2017,45(10):2987-2993,7.基金项目
国家自然科学基金(31260530) （31260530）
