中国畜牧兽医2017，Vol.45Issue(10)：3009-3015,7.DOI:10.16431/j.cnki.1671-7236.2017.10.025
小花棘豆中毒对和田羊母羊繁殖器官的影响
Effects of Oxytropis glabra DC Poisoning on the Reproductive Organs in Hetian Sheep Ewes
摘要
Abstract
The study was aimed to research the effects of Oxytropis glabra DC (O.glabra DC)poisoning on reproductive organs coefficent,reproductive performance and related gene expression in Hetian sheep ewes.The sera,hypothalamus,pituitary and ovary of Hetian sheep ewes cases poisoning of O.glabra DC were collected,the organ exponent and contents of GnRH,FSH,LH,E2 and P4 in serum were measured,then the mRNA expression of reproductive genes were measured.The result demonstrated that the index of hypothalamus,pituitary and ovary were extremely significantly higher than normal sheep (P＜0.01),the follicle number on the surface of the ovaries in poisoning groups were significantly lower than normal sheep (P＜0.05).The result of hematoxylin-eosin staining showed that the nuclear of neuronal cells in hypothalamus were pyknosis and hyperchromatic;The nuclear of cells in pituitary deformation and hyperchromatic,cytoplasm decreased;The primary oocyte in ovary dissolved,disappeared,interstitial blood vessel expanded.The content of GnRH,FSH,LH,E2 and P4 in serum in poisoned Hetian sheep ewes were extremely significantly lower than normal sheep (P＜0.01).The mRNA expression levels of Kiss-1,GPR54,ERα in hypothalamus,GnRHR in pituitary and FSHR and LHR in ovary were extremely significantly lower than normal sheep (P＜0.01).The result showed that O.glabra DC poisoning could affect Hetian sheep ewes reproductive system by affecting microstructure and ultrastructure of hypothalamus-pituitary-ovarian axis.关键词
小花棘豆/中毒/和田羊/繁殖器官Key words
Oxytropis glabra DC/poisoning/Hetian sheep/reproductive organs分类
农业科技引用本文复制引用
姚娜,贾琦珍,陈根元,亚森·麦麦提,王帅..小花棘豆中毒对和田羊母羊繁殖器官的影响[J].中国畜牧兽医,2017,45(10):3009-3015,7.基金项目
国家自然科学基金(31460678) （31460678）
国家星火计划项目(2015GA891015) （2015GA891015）
