H1、H3亚型猪流感病毒双重实时荧光定量RT-PCR检测方法的建立及应用OA北大核心CSTPCD
Development and Application of Duplex Real-time RT-PCR Assay for Detection of H1 and H3 Subtype Swine Influenza Viruses
为建立一种快速、准确地检测H1和H3亚型猪流感病毒(swine influence virus,SIV)的方法,根据H1和H3亚型SIV血凝素(hemagglutinin,HA)基因保守序列,分别设计2对特异性引物和2条TaqMan探针,建立双重实时荧光定量RT-PCR方法.结果显示,该方法敏感性高,可检测到最低拷贝数为102拷贝/μL;重复性良好,重复孔 Ct值的变异系数均在5％以下;特异性好,除H1和H3亚型SIV外,H4、H5、H7、H9亚…查看全部>>
To establish a rapid,accurate method to diagnose and detect H1 and H3 subtype swine influenza viruses (SIV) at the same time,the specific primers and TaqMan probes were designed according to the conserved region of the HA gene of H1 and H3 subtype SIV.A duplex Real-time RT-PCR assay was developed for detection of H1 and H3 subtype SIV.The results showed that the Real-time RT-PCR could detect 102 copies/μL of H1 and H3 subtype SIV,the sensibility was well Coe…查看全部>>
王博;王慧煜;赵宝华;罗静;王承民;何宏轩;韩雪清
中国检验检疫科学研究院,北京100029中国科学院动物研究所,北京100101河北师范大学,石家庄050024中国检验检疫科学研究院,北京100029河北师范大学,石家庄050024中国科学院动物研究所,北京100101中国科学院动物研究所,北京100101
农业科技
H1和H3亚型猪流感病毒(SIV)双重实时荧光定量RT-PCRTaqMan探针
H1 and H3 subtype swine influenza viruses (SIV)duplex Real-time RT-PCRTaq-Man probe
《中国畜牧兽医》 2017 (10)
3035-3041,7
十二五国家科技支撑计划课题(2013BAD12B01)国家林业局野生动物疫源疫病监测项目中国科学院战略生物资源科技支撑体系运行专项野生动物疫源疫病样品组织库(CZBZX-1)
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