中国畜牧兽医2017，Vol.45Issue(10)：3042-3048,7.DOI:10.16431/j.cnki.1671-7236.2017.10.00
鸭腺病毒A型Hexon基因克隆与序列分析
Cloning and Sequence Analysis of Hexon Gene of Duck Adenovirus A
摘要
Abstract
In order to clarify the characteristics of the Hexon gene and the classification candidate of duck adenovirus A under the Adenoviridae family,the Hexon gene fragments of duck adenovirus A (designated as strain JX2016) were amplified.The results demonstrated that the cloned Hexon gene of duck adenovirus A (strain JX2016) was 2 733 bp in length,coding 910 amino acids.Nucleotide homology comparison showed that the strain JX2016 shared the highest nucleotide (99.8％) homology with reference strain FJ12025,also displayed higher than 99.4％ nucleotide homology with other duck adenovirus A reference strains.However,the nucleotide homologies with strain GR (duck adenovirus 2,unclassified virus),Phelps (fowl adenovirus A) and P29 (goose adenovirus) were 54.5％,53.6％ and 55.2％,respectively.The genetic evolution analysis showed that all the duck adenovirus A strains were at the same subgroup,under the same Atadenovirus genus branch with ovine atadenovirus D (strain OAV287).Meanwhile,the duck adenovirus 2 (strain GR) at the same branch under Aviadenovirus genus branch with fowl adenovirus A (strain Phelps) and goose adenovirus (strain P29).Based on the Hexon protein genetic evolution analysis,we suggested renamed the duck adenovirus A as duck-origin atadenovirus and renamed the duck adenovirus 2 as duck-origin aviadenovirus.关键词
鸭腺病毒A型/Hexon基因/序列分析/遗传进化/分类Key words
duck adenovirus A/Hexon gene/sequence analysis/genetic evolution/classification分类
农业科技引用本文复制引用
万春和,刘荣昌,陈翠腾,程龙飞,傅光华,傅秋玲,施少华,陈红梅,黄瑜..鸭腺病毒A型Hexon基因克隆与序列分析[J].中国畜牧兽医,2017,45(10):3042-3048,7.基金项目
国家水禽产业技术体系(CARS-43) （CARS-43）
国家自然科学基金(31602068) （31602068）
福建省农业科学院畜牧兽医研究所基金[MYQJ2015(S)-3] （S）
福建省自然科学基金(2015J01114) （2015J01114）
福建省农业科学院“青年科技英才百人计划”专项(YC2015-12) （YC2015-12）
