中国畜牧兽医2017，Vol.45Issue(10)：3077-3083,7.DOI:10.16431/j.cnki.1671-7236.2017.10.035
水貂源致病性黏质沙雷氏菌的分离鉴定与遗传进化分析
Isolation, Identification and Genetic Evolution Analysis of Mink Serratia marcescens
摘要
Abstract
In order to determine drug resistance,pathogenicity and genetic of the pathogenic bacteria in mink of Heilongjiang province,three strains were isolated from dead minks and identified.Based on colony morphology,microscopic characteristics,biochemical test and 16S rRNA sequencing.The antibiotic susceptibility to 24 antibiotics were detected by using standard K-B paper method,and virulence assay,phylogenetic analysis were carried out at the same time.The result showed that the isolates were highly tolerance to antibacterials,could cause 100％ death of mice in experimental group and were closely related to strains from activated sludge and volcanic deposits.It showed that Serratia marcescens infection of mink existed in Heilongjiang province.Strains resistance phenomenon was serious and had a strong pathogenicity,and might be originated from the environment.It was the first time that the pathogenic Serratia marcescens isolated from the mink was reported.The results provided information for the detection,identification,diagnosis,treatment and prevention of related diseases.关键词
水貂/黏质沙雷氏菌/分离鉴定/遗传进化Key words
mink/Serratia marcescens/isolation and identification/genetic evolution分类
农业科技引用本文复制引用
潘跃,温珊珊,赵丽丽,杨延,李思宇,张乘浩,葛俊伟,陈洪岩..水貂源致病性黏质沙雷氏菌的分离鉴定与遗传进化分析[J].中国畜牧兽医,2017,45(10):3077-3083,7.基金项目
东北农业大学大学生SIPT项目(201710224068) （201710224068）
黑龙江省留学归国人员科学基金项目(LC2015006) （LC2015006）
